Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,223,251	T→C	R121R (CGT→CGC)	panF →	pantothenate:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,223,251	0	T	C	100.0%	257.4 / NA	70	R121R (CGT→CGC)	panF	pantothenate:sodium symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/49);  total (21/49)

GCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGTCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCGATGACCGTGCAGTTTA  >  minE/2223183‑2223318                                                                     |                                                                    gCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGcct                                                                   >  1:3853026/1‑71 (MQ=255) gCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGcct                                                                   >  1:3848123/1‑71 (MQ=255)   gATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGccttc                                                                 <  1:2785442/71‑1 (MQ=255)   gATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGcctt                                                                  >  1:97315/1‑70 (MQ=255)    aTTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGccttct                                                                <  1:1975750/71‑1 (MQ=255)    aTTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGccttc                                                                 <  1:1599786/70‑1 (MQ=255)       cTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGcct                                                                   >  1:3747893/1‑65 (MQ=255)       cTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTgg                                                              <  1:1688571/70‑1 (MQ=255)        ttGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGgtg                                                            >  1:4034333/1‑71 (MQ=255)          gcgcgcCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGgtg                                                            <  1:28465/69‑1 (MQ=255)          gcgcgcCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGgtg                                                            <  1:1070595/69‑1 (MQ=255)          gcgcgcCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGgt                                                             <  1:1300902/68‑1 (MQ=255)          gcgcgcCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGtgtg                                                          >  1:1024525/1‑71 (MQ=255)          gcgcgcCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGtgtg                                                          >  1:726411/1‑71 (MQ=255)           cgcgcCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGgtg                                                            <  1:1872900/68‑1 (MQ=255)           cgcgcCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGtgtg                                                          >  1:322741/1‑70 (MQ=255)             cgccgcTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGtggct                                                       <  1:3907937/71‑1 (MQ=255)              gccgcTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGgt                                                             >  1:3045670/1‑64 (MQ=255)                cgcTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGtggctggc                                                    <  1:1918660/71‑1 (MQ=255)                    aCAATGCAGTGACGCCGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGgtgt                                                           >  1:1607255/1‑60 (MQ=255)                     cAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtt                                               >  1:4022540/1‑71 (MQ=255)                      aaTGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTgg                                                              <  1:503300/55‑1 (MQ=255)                      aaTGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagttt                                              <  1:2726383/71‑1 (MQ=255)                      aaTGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagttt                                              <  1:2714251/71‑1 (MQ=255)                      aaTGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagttt                                              <  1:2612594/71‑1 (MQ=255)                      aaTGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagttt                                              <  1:3948512/71‑1 (MQ=255)                      aaTGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagttt                                              <  1:1232614/71‑1 (MQ=255)                      aaTGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagttt                                              <  1:1300906/71‑1 (MQ=255)                       aTGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttg                                             <  1:3989701/71‑1 (MQ=255)                          cAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttgagt                                          >  1:2273900/1‑71 (MQ=255)                          cAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttgagt                                          <  1:2310275/71‑1 (MQ=255)                          cAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttgagt                                          >  1:1682470/1‑71 (MQ=255)                          cAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttgag                                           <  1:2250485/70‑1 (MQ=255)                            gTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttgagttt                                        <  1:2266183/71‑1 (MQ=255)                             tGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGagtttgagtttg                                       <  1:4046072/71‑1 (MQ=255)                             tGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGagtttgagtttg                                       <  1:128500/71‑1 (MQ=255)                               aCGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGcct                                                                   >  1:2083770/1‑41 (MQ=255)                               aCGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGcct                                                                   <  1:3793574/41‑1 (MQ=255)                                  cTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTg                                                               <  1:187376/42‑1 (MQ=255)                                  cTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGt                                  <  1:542031/71‑1 (MQ=255)                                   tGAACGATATGCTGTTTGCCCGCTACCAGAGTCGcct                                                                   >  1:3400474/1‑37 (MQ=255)                                    gAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTgg                                                              <  1:1122200/41‑1 (MQ=255)                                    gAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTgg                                                              <  1:344789/41‑1 (MQ=255)                                    gAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTgg                                                              <  1:340031/41‑1 (MQ=255)                                    gAACGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGtgtg                                                          >  1:3171898/1‑45 (MQ=255)                                     aaCGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttgagt                                          <  1:542201/60‑1 (MQ=255)                                     aaCGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttgagt                                          <  1:2690595/60‑1 (MQ=255)                                     aaCGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGc                               >  1:3256428/1‑71 (MQ=255)                                      aCGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttgagt                                          <  1:1164119/59‑1 (MQ=255)                                       cGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagttt                                              <  1:2005386/54‑1 (MQ=255)                                       cGATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTg                                >  1:438818/1‑68 (MQ=255)                                        gATATGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGcgtt                            <  1:3192962/71‑1 (MQ=255)                                         atatGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGcgttc                           <  1:127687/71‑1 (MQ=255)                                            tGCTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGcgttcgt                         >  1:2537312/1‑70 (MQ=255)                                              cTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCt                                     <  1:2612197/56‑1 (MQ=255)                                              cTGTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTgg                      <  1:255276/71‑1 (MQ=255)                                                gTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCt                                     <  1:1029085/54‑1 (MQ=255)                                                gTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCt                                     <  1:1126000/54‑1 (MQ=255)                                                gTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCt                                     <  1:2940102/54‑1 (MQ=255)                                                gTTTGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTgg                                   >  1:3212372/1‑56 (MQ=255)                                                 tttGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttga                                            <  1:2026563/46‑1 (MQ=255)                                                 tttGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCgagtttga                                            <  1:3732739/46‑1 (MQ=255)                                                 tttGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGcgtt                            <  1:185882/62‑1 (MQ=255)                                                 tttGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTg                    <  1:2450199/70‑1 (MQ=255)                                                   tGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGGGAGTTTGAGTTTGCTGGTTGcgttc                           >  1:3192666/1‑61 (MQ=255)                                                   tGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCg                  <  1:3932282/70‑1 (MQ=255)                                                   tGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCg                  <  1:1109378/70‑1 (MQ=255)                                                   tGCCCGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCGa                 >  1:1977512/1‑71 (MQ=255)                                                     cccGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCGAt                <  1:3459586/70‑1 (MQ=255)                                                     cccGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCGAt                <  1:1686645/70‑1 (MQ=255)                                                       cGCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTg                                    >  1:2884898/1‑48 (MQ=255)                                                        gCTACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCGATGAcc            <  1:1518337/71‑1 (MQ=255)                                                          tACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTg                                <  1:3224861/49‑1 (MQ=255)                                                          tACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTgg                      <  1:4100795/59‑1 (MQ=255)                                                          tACCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTgg                      <  1:43253/59‑1 (MQ=255)                                                           aacAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGc                                      >  1:3149359/3‑42 (MQ=255)                                                           aCCAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCGATGACCGTg         >  1:3627117/1‑71 (MQ=255)                                                            ccAGAGTCGCCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGcgt                             >  1:1857931/1‑50 (MQ=255)                                                               gagTCGCCTTCTGGTGTGGCTGGCGAGTTTGCGTTTTCTGGtt                                 >  1:2051646/1‑43 (MQ=37)                                                                agTCGCCTTCTGGTGTGGCTGGCGagtttgagtttg                                       <  1:3678873/36‑1 (MQ=255)                                                                 gtcgccTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCGATGACCGTGCAGttt   <  1:3998913/71‑1 (MQ=255)                                                                  tcgccTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCGATGACCGTGCAGTTTa  <  1:3828194/71‑1 (MQ=255)                                                                     |                                                                    GCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGTCTTCTGGTGTGGCTGGCGAGTTTGAGTTTGCTGGTTGCGTTCGTTGGTGCGATGACCGTGCAGTTTA  >  minE/2223183‑2223318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑