| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,349,606 | A→G | V681A (GTG→GCG) | polA ← | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,349,606 | 0 | A | G | 99.0% | 363.5 / ‑5.1 | 98 | V681A (GTG→GCG) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (43/54); minor base T (0/1); total (43/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
CTGAGACAATCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCACCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGCCTGGTGA > minE/2349538‑2349671 | cTGAGACAATCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGcc < 1:174099/71‑1 (MQ=255) tGAGACAATCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCg > 1:2093793/1‑71 (MQ=255) gagaCAATCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCg > 1:4083120/1‑70 (MQ=255) gagaCAATCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCg > 1:1838103/1‑70 (MQ=255) agaCAATCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGCCCTTCTTCGTTACGCGCCgg > 1:2404618/1‑70 (MQ=255) agaCAATCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCgg < 1:3023012/70‑1 (MQ=255) cAATCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATg < 1:1735836/71‑1 (MQ=255) aaTCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGt < 1:3012005/71‑1 (MQ=255) aTCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGtt < 1:1351169/71‑1 (MQ=255) aTCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGtt < 1:2277402/71‑1 (MQ=255) tCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGttt > 1:771981/1‑71 (MQ=255) cacaTAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGtttt > 1:803833/1‑71 (MQ=255) acaTAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTg < 1:3069364/71‑1 (MQ=255) aTAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCa < 1:1297233/71‑1 (MQ=255) aaTCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAgg > 1:3039969/1‑71 (MQ=255) aTCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGt < 1:356985/71‑1 (MQ=255) aTCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGt < 1:378526/71‑1 (MQ=255) ctctGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAg < 1:3279525/71‑1 (MQ=255) ctctGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAg < 1:281207/71‑1 (MQ=255) ctctGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAg < 1:366270/71‑1 (MQ=255) ctGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGtt > 1:939625/1‑58 (MQ=255) ctGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGtt > 1:1893664/1‑58 (MQ=255) gcgcAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGAt < 1:4031059/69‑1 (MQ=255) cAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGtt < 1:2853623/71‑1 (MQ=255) aaTAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCgg > 1:2857255/1‑68 (MQ=255) aaTAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGtt > 1:845551/1‑70 (MQ=255) aaTAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGtt > 1:1442526/1‑70 (MQ=255) aaTAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTg < 1:910742/71‑1 (MQ=255) aaTAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTg < 1:2059245/71‑1 (MQ=255) aaTAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTg < 1:79344/71‑1 (MQ=255) aTAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGtt < 1:1467880/59‑1 (MQ=255) tAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGAc > 1:906947/1‑71 (MQ=255) tAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGAc > 1:3661278/1‑71 (MQ=255) aaCGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTa > 1:1447860/1‑57 (MQ=255) aaCGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACg < 1:2728252/70‑1 (MQ=255) aaCGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACg < 1:3634801/70‑1 (MQ=255) aaCGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGa > 1:1191845/1‑71 (MQ=255) aaCGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGa < 1:3624397/71‑1 (MQ=255) aaCGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGa < 1:2272169/71‑1 (MQ=255) aCGCCTAGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGAt < 1:1842924/71‑1 (MQ=255) cGCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATa < 1:864484/71‑1 (MQ=255) gCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATa > 1:461086/1‑70 (MQ=255) gCCTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATa < 1:62174/70‑1 (MQ=255) cTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATaaa > 1:673832/1‑70 (MQ=255) cTGGCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAAc > 1:2540842/1‑71 (MQ=255) ggCGGATACGACGACCTTCTTCGTTACGCGCCGGGATGTTTTGCAGGTTAGGAt > 1:36408/1‑54 (MQ=39) ggCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGAt > 1:4050374/1‑54 (MQ=255) ggCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAAc > 1:99491/1‑69 (MQ=255) ggCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGt > 1:3309690/1‑71 (MQ=255) gCGGATACGACGCCCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTc > 1:1052747/1‑71 (MQ=255) gCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATaa < 1:1168039/66‑1 (MQ=255) gCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACg < 1:200780/69‑1 (MQ=255) gCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACg < 1:2474006/69‑1 (MQ=255) gCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTc < 1:1458717/71‑1 (MQ=255) gCGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTc > 1:2140502/1‑71 (MQ=255) cGGATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTcc > 1:1448794/1‑71 (MQ=255) ggATACGACGACCTTCTTCGTTACGCGCCGGAATGttt > 1:3427301/1‑38 (MQ=255) ggATACGACGACCTTCTTCGTTACGCGCCGGAATGttt > 1:3753186/1‑38 (MQ=255) ggATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGt < 1:3110253/45‑1 (MQ=255) gATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCg < 1:2147472/71‑1 (MQ=255) gATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCg < 1:2056336/71‑1 (MQ=255) gATACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCg < 1:2983761/71‑1 (MQ=255) tACGACGACCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGtt < 1:3945946/71‑1 (MQ=255) acgacgACCTTCTTCGTTACGCGCCGGAATGTTTTGGAGGTTAGGATCGGTTGACGATAAACGTCCCGTTg > 1:837650/1‑71 (MQ=255) cgacgaCCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTg < 1:1268852/70‑1 (MQ=255) cgacgaCCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTg < 1:377654/70‑1 (MQ=255) cgacgaCCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGc < 1:2069812/71‑1 (MQ=255) gacgacCTTCTTCGTTACGCTCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGAt < 1:3457239/56‑1 (MQ=255) gacgacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCg < 1:2242754/47‑1 (MQ=255) gacgacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTg > 1:291859/1‑69 (MQ=255) gacgacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCa < 1:2383162/71‑1 (MQ=255) acgacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAAc > 1:1885003/1‑59 (MQ=255) acgacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAAc > 1:341595/1‑59 (MQ=255) cgacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAg > 1:3544794/1‑70 (MQ=255) cgacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGt > 1:2909852/1‑71 (MQ=255) cgacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGt > 1:455152/1‑71 (MQ=255) cgacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGt > 1:3640486/1‑71 (MQ=255) gacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGt < 1:3680178/64‑1 (MQ=255) gacCTTCTTCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGtt < 1:2196373/71‑1 (MQ=255) cttcttCGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACt > 1:2046155/1‑71 (MQ=255) ttcttcGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTg > 1:4032738/1‑71 (MQ=255) ttcttcGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTg > 1:151426/1‑71 (MQ=255) cttcGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTg < 1:3853243/69‑1 (MQ=255) cttcGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGcc > 1:3478680/1‑71 (MQ=255) ttcGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGtt < 1:1322514/40‑1 (MQ=255) ttcGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGa > 1:3755353/1‑42 (MQ=255) cGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGcc < 1:3039001/68‑1 (MQ=255) cGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGcc < 1:4048005/68‑1 (MQ=255) cGTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGCCt < 1:1312538/69‑1 (MQ=255) gTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGcc < 1:205866/67‑1 (MQ=255) gTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGcc < 1:3127172/67‑1 (MQ=255) gTTACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGCCTGGt < 1:3938813/71‑1 (MQ=255) ttACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATaa < 1:447652/44‑1 (MQ=255) ttACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATaa < 1:3060805/44‑1 (MQ=255) ttACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGCCTGGTg < 1:3518342/71‑1 (MQ=255) ttACGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGCCTGGTg < 1:854743/71‑1 (MQ=255) aCGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGCCTGGTGa > 1:2946954/1‑70 (MQ=255) aCGCGCCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGCCTGGTGa > 1:2160204/1‑70 (MQ=255) | CTGAGACAATCACATAATCCTCTGGCGCAATAAACGCCTGGCGGATACGACGACCTTCTTCGTTACGCACCGGAATGTTTTGCAGGTTAGGATCGGTTGACGATAAACGTCCCGTTGCAGTTACTGCCTGGTGA > minE/2349538‑2349671 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |