Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,364,370	T→C	N367D (AAC→GAC)	trkH ←	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,364,370	0	T	C	100.0%	177.8 / NA	49	N367D (AAC→GAC)	trkH	potassium transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/22);  total (27/22)

CGCGATTCCCCAGCTTAATGCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTTCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGTTGACCCGGCGCAA  >  minE/2364307‑2364434                                                                |                                                                 cgcgATTCCCCAGCTTAATGCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGc                                                           >  1:832232/1‑71 (MQ=255) cgcgATTCCCCAGCTTAATGCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGc                                                           <  1:1852314/71‑1 (MQ=255)    gATTCCCCAGCTTAATGCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCt                                                             >  1:558591/1‑66 (MQ=255)    gATTCCCCAGCTTAATGCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTa                                                        <  1:1972467/71‑1 (MQ=255)            aGCTTAATGCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAAcag                                                   >  1:1858213/1‑68 (MQ=255)                  aTGCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGa                                           >  1:517858/1‑70 (MQ=255)                   tGCTATAGACGGCGTGCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATg                                         <  1:324170/71‑1 (MQ=255)                    gCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAAcagca                                                 >  1:893353/1‑62 (MQ=255)                    gCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGc                                        >  1:3061621/1‑71 (MQ=255)                     cTATAGACGGCGTTCGGATGCACCACTCGTTTCAGCTCACGGTCCCCCTGCTTaa                                                       <  1:2041137/55‑1 (MQ=255)                        taGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCgg                                      <  1:3807607/69‑1 (MQ=255)                          gACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATc                                   <  1:707874/70‑1 (MQ=255)                            cGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCAc                                 >  1:502934/1‑70 (MQ=255)                             ggCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAAcagcagcag                                             <  1:3319871/57‑1 (MQ=255)                             ggCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATc                                   >  1:836719/1‑67 (MQ=255)                             ggCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATc                                   >  1:3101010/1‑67 (MQ=255)                             ggCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCt                               <  1:1015775/71‑1 (MQ=255)                              gCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAAcagca                                                 >  1:2372302/1‑52 (MQ=255)                               cGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGCTCACCTTc                             <  1:1776698/71‑1 (MQ=255)                               cGTTCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTc                             <  1:3268997/71‑1 (MQ=255)                                 ttCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTaaa                                                      <  1:2926404/44‑1 (MQ=255)                                 ttCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAg                           <  1:448807/71‑1 (MQ=255)                                  tCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGc                                        >  1:1041229/1‑57 (MQ=255)                                  tCGGATGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGc                                        >  1:2166233/1‑57 (MQ=255)                                       tGCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAAcag                                                   >  1:760724/1‑41 (MQ=255)                                        gCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCcccc                     >  1:1916596/1‑70 (MQ=255)                                        gCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCg                    >  1:3869576/1‑71 (MQ=255)                                        gCACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCg                    >  1:193255/1‑71 (MQ=255)                                         cACCAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGc                   <  1:3047098/71‑1 (MQ=255)                                           ccAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGAt                                    >  1:3037955/1‑52 (MQ=255)                                           ccAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCt                               <  1:2954934/57‑1 (MQ=255)                                            cAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAAcagcagc                                               >  1:1008565/1‑40 (MQ=255)                                            cAGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATc                                   <  1:3617123/52‑1 (MQ=255)                                             aGTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGt               >  1:3586385/1‑71 (MQ=255)                                              gTCGTTTCAGCTCACGTTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGtt              >  1:2392137/1‑71 (MQ=255)                                              gTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGtt              >  1:3731269/1‑71 (MQ=255)                                              gTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGtt              <  1:1392604/71‑1 (MQ=255)                                              gTCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCCtt              >  1:1943297/1‑71 (MQ=255)                                               tCGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGa                                     >  1:2602540/1‑47 (MQ=255)                                                cGTTTCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGccc                 <  1:650834/66‑1 (MQ=255)                                                  tttCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGTTGAcc          <  1:2468497/71‑1 (MQ=255)                                                  tttCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGTTGAcc          <  1:1677958/71‑1 (MQ=255)                                                    tCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGtt              >  1:1816150/1‑65 (MQ=255)                                                    tCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGTTGACCCg        >  1:2320261/1‑71 (MQ=255)                                                    tCAGCTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCACGtt              >  1:3903113/1‑65 (MQ=255)                                                        cTCACGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGTTGACCCGgcgc    <  1:1241502/71‑1 (MQ=255)                                                           aCGGTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGTTGACCCGGCGCa   <  1:60789/69‑1 (MQ=255)                                                             ggTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGTTGAcc          <  1:2527182/60‑1 (MQ=255)                                                             ggTCCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGTTGACCCGGCGCaa  >  1:2077270/1‑68 (MQ=255)                                                                |                                                                 CGCGATTCCCCAGCTTAATGCTATAGACGGCGTTCGGATGCACCAGTCGTTTCAGCTCACGGTTCCCCTGCTTAAACAGCAGCAGGATGCGGATCACCTTCAGGCCACCGCCCGTTGACCCGGCGCAA  >  minE/2364307‑2364434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑