Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,370,014	G→A	V58V (GTG→GTA)	fadA →	3‑ketoacyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,370,014	0	G	A	100.0%	129.6 / NA	42	V58V (GTG→GTA)	fadA	3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/22);  total (20/22)

CGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTGGCAGAAGTA  >  minE/2369946‑2370074                                                                     |                                                             cGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTTTGTaca                                                            <  1:8323/71‑1 (MQ=255)   tAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTacag                                                           >  1:1295838/1‑70 (MQ=255)     gCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTacag                                                           >  1:3523726/1‑68 (MQ=255)     gCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTacag                                                           >  1:2912169/1‑68 (MQ=255)       ctgctgGCGCGTAACCCGGCTCTGGAAGCGGCGGCCGGCGACGATATTTACTGGGGTTGTGTACAGCAGAc                                                      >  1:1591142/1‑71 (MQ=255)                 gTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAg                                            >  1:2110173/1‑71 (MQ=255)                    aCCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAggg                                          <  1:2955251/70‑1 (MQ=255)                    aCCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGt                                         >  1:3810344/1‑71 (MQ=255)                       cGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGtttt                                      <  1:99239/71‑1 (MQ=255)                       cGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGtttt                                      <  1:474130/71‑1 (MQ=255)                       cGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGttt                                       >  1:2131429/1‑70 (MQ=255)                          cgcTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAat                                   <  1:257622/71‑1 (MQ=255)                          cgcTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAat                                   <  1:2137368/71‑1 (MQ=255)                           gcTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAata                                  <  1:187489/71‑1 (MQ=255)                               gAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGc                              >  1:2790704/1‑71 (MQ=255)                                    ggcggcCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAg                                            >  1:3230296/1‑52 (MQ=255)                                    ggcggcCCTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGt                          >  1:710631/1‑70 (MQ=255)                                         cccTCGACTATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCa                                             <  1:778000/46‑1 (MQ=255)                                         cccTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTg                                                  <  1:2115977/41‑1 (MQ=255)                                         cccTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTg                                                  <  1:2038056/41‑1 (MQ=255)                                           cTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTgg                                                 <  1:1225480/40‑1 (MQ=255)                                           cTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTgg                                                 <  1:1610845/40‑1 (MQ=255)                                           cTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGa                                                >  1:1963633/1‑41 (MQ=255)                                           cTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAat                                   >  1:3934051/1‑54 (MQ=255)                                           cTCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACgcggc                  <  1:3157185/71‑1 (MQ=255)                                            tCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGttt                                       >  1:3216935/1‑49 (MQ=255)                                            tCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAat                                   <  1:2808348/53‑1 (MQ=255)                                            tCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACgcggcg                 >  1:2070601/1‑71 (MQ=255)                                            tCGACGATATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAAAgcggcg                 >  1:1685839/1‑71 (MQ=255)                                             cgacgaTATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAg                                            <  1:3688903/43‑1 (MQ=255)                                             cgacgaTATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGgcgc                >  1:264298/1‑71 (MQ=255)                                              gacgaTATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTaa                                    >  1:1548290/1‑50 (MQ=255)                                              gacgaTATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCgct               >  1:1177510/1‑71 (MQ=255)                                                cgaTATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCgctgc             <  1:3837453/71‑1 (MQ=255)                                                cgaTATTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCgctgc             >  1:1418815/1‑71 (MQ=255)                                                 gaTATATACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCgctgct            >  1:2258276/1‑71 (MQ=255)                                                  atatTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGctgctg           <  1:3558236/71‑1 (MQ=255)                                                  atatTTACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGctgctg           >  1:2284035/1‑71 (MQ=255)                                                     tttACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTaa                        >  1:415227/1‑55 (MQ=255)                                                     tttACTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTgg          >  1:513171/1‑69 (MQ=255)                                                        aCTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTGGCAGa      <  1:2983691/70‑1 (MQ=255)                                                         cTGGGGTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTGGCAGAAg    <  1:2288305/71‑1 (MQ=255)                                                            gggTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTGGCAGAAGt   <  1:3603856/69‑1 (MQ=255)                                                            gggTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTGGCAGAAGt   <  1:73112/69‑1 (MQ=255)                                                            gggTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTGGCAGAAGt   <  1:1250472/69‑1 (MQ=255)                                                            gggTTGTGTACAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTGGCAGAAGTa  <  1:184003/70‑1 (MQ=255)                                                                     |                                                             CGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGTTTTAATATCGCCCGTAACGCGGCGCTGCTGGCAGAAGTA  >  minE/2369946‑2370074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑