| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,415,011 | A→G | L215L (CTT→CTC) | aslB ← | predicted regulator of arylsulfatase activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,415,011 | 0 | A | G | 98.2% | 206.9 / NA | 56 | L215L (CTT→CTC) | aslB | predicted regulator of arylsulfatase activity |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/35); minor base T (0/1); total (20/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAACTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTAAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGAATTC > minE/2414944‑2415076 | cGAACTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGt > 1:1934181/1‑71 (MQ=255) aaCTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTcc < 1:3512572/71‑1 (MQ=255) aCTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCa < 1:109030/71‑1 (MQ=255) aCTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCa < 1:337113/71‑1 (MQ=255) tcGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTcc < 1:320284/65‑1 (MQ=255) cGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg > 1:1175205/1‑61 (MQ=255) cGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGAATGTAACTTGAGTCCATCGCgg > 1:2319976/1‑71 (MQ=255) gcgcACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCa < 1:1976246/61‑1 (MQ=255) gcACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAgtt < 1:2905159/71‑1 (MQ=255) cACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAgttg < 1:957465/71‑1 (MQ=255) ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg > 1:981466/1‑52 (MQ=255) ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg > 1:1195578/1‑52 (MQ=255) ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg > 1:123291/1‑52 (MQ=255) ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGt < 1:1312792/53‑1 (MQ=255) ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAgttgtt < 1:1555977/71‑1 (MQ=255) cGACCATTCCGTTATCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg < 1:1773958/51‑1 (MQ=255) cGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGttt > 1:1184129/1‑71 (MQ=255) gACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGttt > 1:2912212/1‑70 (MQ=255) aCCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCggg > 1:258618/1‑60 (MQ=255) aCCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTtca < 1:3549946/71‑1 (MQ=255) ccATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTtcat < 1:1805925/71‑1 (MQ=255) ttCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAg > 1:2679982/1‑71 (MQ=255) cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTtca < 1:3182468/64‑1 (MQ=255) cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGcc < 1:2150174/70‑1 (MQ=255) cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGcc < 1:913180/70‑1 (MQ=255) cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGcc < 1:855948/70‑1 (MQ=255) cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCa < 1:2444435/71‑1 (MQ=255) gTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCTTCAGCCAg < 1:917803/71‑1 (MQ=255) ttAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAgg < 1:2890889/71‑1 (MQ=255) aGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGgc < 1:3029048/70‑1 (MQ=255) cTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAgttgt < 1:1578039/54‑1 (MQ=255) aCCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTc < 1:917672/69‑1 (MQ=255) aCCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTc < 1:2105408/69‑1 (MQ=255) aCCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTc < 1:4140029/69‑1 (MQ=255) cccTGAATATCACCAGGCGCATGTAACTTGAGTcc < 1:4213/35‑1 (MQ=255) cccTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGac > 1:3452223/1‑71 (MQ=255) gAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGcc > 1:132425/1‑56 (MQ=255) gAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGaccacc > 1:1688366/1‑71 (MQ=255) gAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGa > 1:1457153/1‑66 (MQ=255) aaTATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGaccac < 1:1304318/69‑1 (MQ=255) aaTATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGCCCACCg > 1:434445/1‑71 (MQ=255) atatCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTTTTTCATCAGCCAGGCGCTCGACCACCgg < 1:2591342/71‑1 (MQ=255) tCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGaa > 1:2252280/1‑70 (MQ=255) cACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAAt > 1:3314602/1‑70 (MQ=255) aCCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATaa < 1:3054316/71‑1 (MQ=255) aCCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATa > 1:4127104/1‑70 (MQ=255) ccAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGac > 1:3843034/1‑59 (MQ=255) ggCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTtcat > 1:1540493/1‑40 (MQ=255) ggCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTg < 1:1044763/71‑1 (MQ=255) gcgcATGTAACTTTAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGgcg < 1:2129018/49‑1 (MQ=255) gcgcATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTgg < 1:3471846/71‑1 (MQ=255) aTGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGa < 1:833568/71‑1 (MQ=255) tGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGc < 1:3390977/38‑1 (MQ=255) tGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGaa < 1:779468/71‑1 (MQ=255) tGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGaa < 1:3249919/71‑1 (MQ=255) aCTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGAATTc < 1:1170250/70‑1 (MQ=255) | CGAACTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTAAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGAATTC > minE/2414944‑2415076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |