Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	131,828	C→T	L41F (CTC→TTC)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	131,828	0	C	T	100.0%	290.6 / NA	92	L41F (CTC→TTC)	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/46);  total (46/46)

TGCATGCGGAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACCTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACGGACGT  >  minE/131760‑131895                                                                     |                                                                    tgcatgcGGAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTc                                                                   <  1:3624369/71‑1 (MQ=255)   catgcGGAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCaa                                                                 >  1:2136439/1‑71 (MQ=255)   catgcGGAGACGGCTCCCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCa                                                                  <  1:3766625/70‑1 (MQ=255)    atgcGGAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCaa                                                                 >  1:2026486/1‑70 (MQ=255)    atgcGGAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAac                                                                <  1:1210634/71‑1 (MQ=255)      gcGGAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAacac                                                              >  1:2022539/1‑71 (MQ=255)      gcGGAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAacac                                                              >  1:3846896/1‑71 (MQ=255)       cGGAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAaca                                                               >  1:4030655/1‑69 (MQ=255)        ggAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGc                                                            <  1:1562008/71‑1 (MQ=255)           gaCGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTc                                                         <  1:4013857/71‑1 (MQ=255)              ggCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCAtt                                                      <  1:3652113/71‑1 (MQ=255)                cTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCAtt                                                      >  1:370021/1‑69 (MQ=255)                cTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCAtt                                                      >  1:558576/1‑69 (MQ=255)                   gcgACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTagag                                                 <  1:2105124/71‑1 (MQ=255)                   gcgACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTaga                                                  >  1:4033941/1‑70 (MQ=255)                    cgACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGt                                                <  1:3559687/71‑1 (MQ=255)                       cTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTc                                               <  1:3827533/69‑1 (MQ=255)                          tCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCggg                                            <  1:780088/69‑1 (MQ=255)                          tCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCggg                                            <  1:60815/69‑1 (MQ=255)                          tCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCggg                                            >  1:988420/1‑69 (MQ=255)                            tGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCt                                                           <  1:637828/52‑1 (MQ=255)                            tGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTc                                                         <  1:3162916/54‑1 (MQ=255)                              gagaTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCa                                                                  <  1:1729151/43‑1 (MQ=255)                              gagaTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCa                                                                  <  1:2119176/43‑1 (MQ=255)                              gagaTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCa                                                                  <  1:3418026/43‑1 (MQ=255)                              gagaTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGttt                                      <  1:112929/71‑1 (MQ=255)                                gaTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCa                                    >  1:2911439/1‑71 (MQ=255)                                 aTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTa                                                    <  1:682805/54‑1 (MQ=255)                                 aTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTa                                                    <  1:1716123/54‑1 (MQ=255)                                 aTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCgggg                                           <  1:1753539/63‑1 (MQ=255)                                 aTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCgggg                                           <  1:2368109/63‑1 (MQ=255)                                 aTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCAt                                   >  1:1988511/1‑71 (MQ=255)                                 aTACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCAt                                   >  1:1364512/1‑71 (MQ=255)                                  tACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCAtt                                  >  1:98071/1‑71 (MQ=255)                                  tACATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCAtt                                  <  1:3881605/71‑1 (MQ=255)                                  tACATTTGCCAGTGGAATTACGCGAACTTTGAGACTTCAACACGCTTCATTTaga                                                  >  1:1698247/1‑55 (MQ=255)                                   aCATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCAtt                                  >  1:3459742/1‑70 (MQ=255)                                    cATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTg                                 <  1:2284992/70‑1 (MQ=255)                                    cATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTg                                 <  1:2925589/70‑1 (MQ=255)                                    cATTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGa                                <  1:2954044/71‑1 (MQ=255)                                     aTTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTg                                 >  1:918249/1‑69 (MQ=255)                                     aTTTGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTg                                 <  1:2737227/69‑1 (MQ=255)                                      tttGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCgg                                             <  1:1474348/56‑1 (MQ=255)                                      tttGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAag                              <  1:1326434/71‑1 (MQ=255)                                       ttGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTa                                                    >  1:2585587/1‑48 (MQ=255)                                       ttGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGaa                               >  1:1623873/1‑69 (MQ=255)                                        tGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTa                                                    >  1:819791/1‑47 (MQ=255)                                        tGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCggg                                            >  1:2146108/1‑55 (MQ=255)                                        tGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCgg                                             >  1:566338/1‑54 (MQ=255)                                        tGCCAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCAtt                                  >  1:3200251/1‑65 (MQ=255)                                         gCCAGTGGAATTACGCGAACTTTGTGACTTCAACTCGCTTCATTTAGAGTCGGGGAGTTTCATTGAagag                            <  1:2065341/70‑1 (MQ=255)                                          ccAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAagaga                           <  1:120619/70‑1 (MQ=255)                                          ccAGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAagag                            <  1:971562/69‑1 (MQ=255)                                            aGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCAt                                                       >  1:994186/1‑40 (MQ=255)                                            aGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTa                                                    >  1:2750229/1‑43 (MQ=255)                                            aGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTa                                                    >  1:2933826/1‑43 (MQ=255)                                            aGTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTa                                                    >  1:2165322/1‑43 (MQ=255)                                             gTGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCt                       >  1:651211/1‑71 (MQ=255)                                              tGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTa                                                    <  1:3244627/41‑1 (MQ=255)                                              tGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCggg                                            <  1:3986754/49‑1 (MQ=255)                                              tGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTg                                 <  1:2213371/60‑1 (MQ=255)                                              tGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCt                       >  1:1182243/1‑70 (MQ=255)                                              tGGAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCt                       >  1:2538784/1‑70 (MQ=255)                                               ggAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTc                                               <  1:2327599/45‑1 (MQ=255)                                                gAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGa                     <  1:6373/70‑1 (MQ=255)                                                gAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGa                     <  1:1363645/70‑1 (MQ=255)                                                gAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGa                     <  1:3956895/70‑1 (MQ=255)                                                gAATTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGa                     >  1:347402/1‑70 (MQ=255)                                                  aTTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGaa                               >  1:3146493/1‑58 (MQ=255)                                                  aTTACGCGAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAg                  >  1:2844516/1‑71 (MQ=255)                                                      cgcgAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGaca              <  1:4033606/71‑1 (MQ=255)                                                       gcgAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGttt                                      <  1:2289140/46‑1 (MQ=255)                                                       gcgAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGGCTGAAAGGaca              >  1:1214438/1‑70 (MQ=255)                                                        cgAACTTTGTGACTTCAACACGCTTCATTTAGAGTCggg                                            >  1:283854/1‑39 (MQ=255)                                                         gAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTg                                 <  1:69252/49‑1 (MQ=255)                                                         gAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTg                                 <  1:1122032/49‑1 (MQ=255)                                                         gAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGa                     <  1:3916333/61‑1 (MQ=255)                                                         gAACTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAg           <  1:1217629/71‑1 (MQ=255)                                                          aaCTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGc          <  1:3833489/71‑1 (MQ=255)                                                           aCTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGa                                          >  1:1665572/1‑38 (MQ=255)                                                           aCTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAg                                         >  1:2966216/1‑39 (MQ=255)                                                           aCTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCa                                    >  1:3054360/1‑44 (MQ=255)                                                           aCTTTGTGACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTAAt                                   >  1:4146437/1‑45 (MQ=255)                                                               tgtgACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTGATTGAAGAGAGCCt                       >  1:3370338/1‑53 (MQ=255)                                                               tgtgACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACg       >  1:2135101/1‑69 (MQ=255)                                                               tgtgACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACGGa     >  1:2441764/1‑71 (MQ=255)                                                               tgtgACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACGGa     >  1:3633080/1‑71 (MQ=255)                                                               tgtgACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACGGa     >  1:3352919/1‑71 (MQ=255)                                                               tgtgACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACGGa     >  1:3386275/1‑71 (MQ=255)                                                                gtgACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACGGAc    >  1:3090522/1‑71 (MQ=255)                                                                 tgACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACGGAc    <  1:1211617/70‑1 (MQ=255)                                                                  gACTTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACGGACGt  >  1:2808749/1‑71 (MQ=255)                                                                     |                                                                    TGCATGCGGAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGACCTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGCACGGACGT  >  minE/131760‑131895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑