Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,478,588	C→T	I187I (ATC→ATT)	pstB →	phosphate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,478,588	0	C	T	100.0%	280.6 / NA	88	I187I (ATC→ATT)	pstB	phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (52/36);  total (52/36)

CGCGTGGTATCGCCATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATCTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGATCGTCACC  >  minE/2478521‑2478654                                                                    |                                                                   cgcgTGGTATCGCCATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATttct                                                                 <  1:2003096/71‑1 (MQ=255)    gTGGTATCGCCATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTAcc                                                              <  1:3542288/71‑1 (MQ=255)      ggTATCGCCATTCGCCCGTAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCgg                                                            >  1:3969090/1‑71 (MQ=255)         aTCGCCATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTa                                                                <  1:1339806/64‑1 (MQ=255)          tCGCCATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCg                                                         >  1:1141930/1‑70 (MQ=255)          tCGCCATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCg                                                         <  1:1462653/70‑1 (MQ=255)           cGCCATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTa                                                       >  1:2050907/1‑71 (MQ=255)            gCCATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTa                                                       <  1:203361/70‑1 (MQ=255)             ccATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTAtt                                                     <  1:1649785/71‑1 (MQ=255)                  cGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGaa                                                  >  1:3441498/1‑69 (MQ=255)                     ccGGAAGTGCTTCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGt                                                        <  1:2340917/60‑1 (MQ=39)                     ccGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCg                                                             >  1:2257966/1‑55 (MQ=255)                     ccGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAag                                                 <  1:2773596/67‑1 (MQ=255)                     ccGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGc                                              >  1:1714134/1‑70 (MQ=255)                     ccGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCt                                             <  1:3527958/71‑1 (MQ=255)                       ggAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTg                                                    <  1:1014795/62‑1 (MQ=255)                       ggAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAaga                                                >  1:1299519/1‑66 (MQ=255)                       ggAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGa                                           >  1:824066/1‑71 (MQ=255)                        gAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGaa                                                  >  1:1731716/1‑63 (MQ=255)                           gTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCAc                                       <  1:3299132/71‑1 (MQ=255)                           gTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCAc                                       >  1:1247843/1‑71 (MQ=255)                              ctgctgctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAagag                                               >  1:152653/1‑60 (MQ=255)                               tgctgctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGaa                                   >  1:3972143/1‑71 (MQ=255)                                gctgctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAAc                                  >  1:906404/1‑71 (MQ=255)                                 ctgctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACt                                 >  1:252293/1‑71 (MQ=255)                                 ctgctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACt                                 >  1:899968/1‑71 (MQ=255)                                 ctgctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACt                                 >  1:1423679/1‑71 (MQ=255)                                  tgctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACt                                 >  1:3046219/1‑70 (MQ=255)                                  tgctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACt                                 >  1:3105171/1‑70 (MQ=255)                                  tgctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACt                                 >  1:1144272/1‑70 (MQ=255)                                    ctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTAtt                                                     <  1:1838112/48‑1 (MQ=255)                                    ctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTg                                <  1:3334601/69‑1 (MQ=255)                                    ctCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGaa                              <  1:1781016/71‑1 (MQ=255)                                     tCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCgg                                                            >  1:2254263/1‑40 (MQ=255)                                     tCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGa                                    >  1:288661/1‑64 (MQ=255)                                     tCGACGAACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAg                             >  1:1282468/1‑71 (MQ=255)                                       gacgaACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGaa                                                  >  1:3327085/1‑48 (MQ=255)                                       gacgaACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAg                             >  1:4058527/1‑69 (MQ=255)                                       gacgaACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAg                             >  1:1764464/1‑69 (MQ=255)                                       gacgaACCGTGTTCGGCGCT‑‑ACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAg                          >  1:1707194/1‑70 (MQ=38)                                        acgaACAGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAg                          >  1:537521/1‑71 (MQ=255)                                         cgaACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAg                          >  1:2270260/1‑70 (MQ=255)                                          gaACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGa                        >  1:3199011/1‑71 (MQ=255)                                          gaACCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGa                        >  1:678911/1‑71 (MQ=255)                                           aaCCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTg                                            <  1:2738024/50‑1 (MQ=255)                                           aaCCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGAt                       <  1:3532043/71‑1 (MQ=255)                                           aaCCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGAt                       <  1:2037541/71‑1 (MQ=255)                                           aaCCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGAt                       <  1:1933008/71‑1 (MQ=255)                                            aCCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCAcc                                      >  1:2091832/1‑55 (MQ=255)                                            aCCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGAtt                      <  1:3611846/71‑1 (MQ=255)                                            aCCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGAtt                      <  1:3234944/71‑1 (MQ=255)                                            aCCGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGAt                       <  1:1206254/70‑1 (MQ=255)                                             ccGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTg                                                    <  1:4106838/40‑1 (MQ=255)                                             ccGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTa                     <  1:3015431/71‑1 (MQ=255)                                              cGTGTTCGGCGCTCGCCCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTa                     >  1:1022995/1‑70 (MQ=255)                                              cGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGa                                                   >  1:3087540/1‑40 (MQ=255)                                              cGTGTTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGa                                                   >  1:3598987/1‑40 (MQ=255)                                               gtgtTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCt                                             <  1:1657133/45‑1 (MQ=255)                                               gtgtTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTaca                   >  1:28922/1‑71 (MQ=255)                                               gtgtTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTaca                   >  1:2467766/1‑71 (MQ=255)                                                tgtTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTacac                  >  1:567332/1‑71 (MQ=255)                                                 gtTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTacac                  >  1:1312890/1‑70 (MQ=255)                                                 gtTCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACAcc                 >  1:2257172/1‑71 (MQ=255)                                                  ttCGGCGCTCGACCCTATTTCTACCGGGCGTATTg                                                    <  1:1234073/35‑1 (MQ=255)                                                  ttCGGCGCTCGACCCTATTTCTACCGGGCGTATTGaa                                                  >  1:2935609/1‑37 (MQ=255)                                                  ttCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCg                >  1:1011052/1‑71 (MQ=255)                                                  ttCGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCg                <  1:3629702/71‑1 (MQ=255)                                                    cGGCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGaa                                   >  1:1849814/1‑50 (MQ=255)                                                     ggCGCTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCgtg              <  1:1889624/70‑1 (MQ=255)                                                       cgcTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATTACCGAACt                                 <  1:3559436/49‑1 (MQ=38)                                                       cgcTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCgtggtg           <  1:1499030/71‑1 (MQ=255)                                                        gcTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGa          <  1:3996727/71‑1 (MQ=255)                                                        gcTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGa          >  1:3160197/1‑71 (MQ=255)                                                         cTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGaa                                   >  1:2091298/1‑45 (MQ=255)                                                         cTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGa          <  1:1612961/70‑1 (MQ=255)                                                         cTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGa          <  1:3660469/70‑1 (MQ=255)                                                         cTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGAt         <  1:2771701/71‑1 (MQ=255)                                                         cTCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGAt         >  1:639802/1‑71 (MQ=255)                                                          tCGACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGATc        >  1:1152329/1‑71 (MQ=255)                                                            gACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGATc        <  1:1541652/69‑1 (MQ=255)                                                            gACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGATCGt      >  1:2692821/1‑71 (MQ=255)                                                            gACCCTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGATCGt      <  1:789970/71‑1 (MQ=255)                                                              cccTATTTCTACCGGGGGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGATCGTCa    >  1:643114/1‑71 (MQ=255)                                                               ccTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGATCGTCAc   >  1:3999106/1‑71 (MQ=255)                                                                cTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGa          >  1:2245560/1‑63 (MQ=255)                                                                cTATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGa          >  1:3557728/1‑63 (MQ=255)                                                                 tATTTCTACCGGGCGTATTGAAGAGCTGATTACCGAACTGAAGCAGGATTACACCGTGGTGATCGTCAcc  >  1:637689/1‑70 (MQ=255)                                                                 tATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGaa                              <  1:885132/42‑1 (MQ=255)                                                                 tATTTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTAAAGCAGGATTaca                   <  1:4088005/53‑1 (MQ=38)                                                                    |                                                                   CGCGTGGTATCGCCATTCGCCCGGAAGTGCTGCTGCTCGACGAACCGTGTTCGGCGCTCGACCCTATCTCTACCGGGCGTATTGAAGAGCTGATCACCGAACTGAAGCAGGATTACACCGTGGTGATCGTCACC  >  minE/2478521‑2478654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑