Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,697,811	G→A	R139C (CGT→TGT)	mrcA ←	fused penicillin‑binding protein 1a murein transglycosylase and murein transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,697,811	0	G	A	100.0%	279.2 / NA	88	R139C (CGT→TGT)	mrcA	fused penicillin‑binding protein 1a murein transglycosylase and murein transpeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/46);  total (42/46)

GAGGATCTCGTCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACGCATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATGGTACTTGC  >  minE/2697746‑2697874                                                                  |                                                                gAGGATCTCGTCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACAt                                                              >  1:801620/1‑69 (MQ=255) gAGGATCTCGTCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACAt                                                              >  1:3942121/1‑69 (MQ=255) gAGGATCTCGTCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACAt                                                              >  1:3946687/1‑69 (MQ=255)   ggATCTCGTCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAgc                                                          >  1:3518534/1‑71 (MQ=255)         cGTCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAgcgtgcgt                                                    >  1:4059439/1‑71 (MQ=255)          gTCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGtt                                                   >  1:2822115/1‑71 (MQ=255)          gTCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGtt                                                   >  1:2882619/1‑71 (MQ=255)           tCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGtt                                                   <  1:2798079/70‑1 (MQ=255)           tCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTc                                                  <  1:347667/71‑1 (MQ=255)            cTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCt                                                 >  1:3551091/1‑71 (MQ=255)            cTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCt                                                 >  1:1565911/1‑71 (MQ=255)             tttCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTg                                                >  1:2854813/1‑71 (MQ=255)             tttCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGAATTCCTTAATCTTACACATCAGCGTGCGTTCTg                                                >  1:491123/1‑71 (MQ=255)               tCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAgcgt                                                        >  1:3282914/1‑61 (MQ=255)               tCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTgg                                               <  1:1971388/70‑1 (MQ=255)               tCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTg                                                <  1:2798719/69‑1 (MQ=255)                 gTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGAc                                             <  1:850347/70‑1 (MQ=255)                 gTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACt                                            <  1:285669/71‑1 (MQ=255)                    agcagcTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGa                                          <  1:124936/70‑1 (MQ=255)                    agcagcTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAg                                         >  1:900281/1‑71 (MQ=255)                       agcTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAgg                                        >  1:1016394/1‑69 (MQ=255)                        gcTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGtt                                                   <  1:3711553/57‑1 (MQ=255)                        gcTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAg                                         >  1:2611454/1‑67 (MQ=255)                         cTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAgcgtgcgt                                                    <  1:3449771/55‑1 (MQ=255)                         cTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGgaag                                     >  1:1544106/1‑70 (MQ=255)                          tGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGgaag                                     >  1:3889235/1‑69 (MQ=255)                          tGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGgaag                                     >  1:2647210/1‑69 (MQ=255)                            ttCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGgaagaa                                   <  1:2464281/69‑1 (MQ=255)                            ttCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGaagaag                                  <  1:2628334/70‑1 (MQ=255)                            ttCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGt                                 <  1:1317928/71‑1 (MQ=255)                            ttCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGt                                 <  1:1999567/71‑1 (MQ=255)                            ttCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGt                                 <  1:3766204/71‑1 (MQ=255)                            ttCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGt                                 <  1:1830940/71‑1 (MQ=255)                            ttCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGt                                 <  1:1653561/71‑1 (MQ=255)                             tCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGtt                                >  1:1049314/1‑71 (MQ=255)                             tCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGt                                 >  1:709417/1‑70 (MQ=255)                              cAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGttt                               >  1:2288244/1‑71 (MQ=255)                               aaTGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAgcgtgcgt                                                    <  1:1892822/49‑1 (MQ=255)                               aaTGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGttt                               >  1:766918/1‑70 (MQ=255)                               aaTGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGttt                               >  1:3574122/1‑70 (MQ=255)                                aTGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGa                                              >  1:781272/1‑54 (MQ=255)                                aTGCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGa                                              >  1:3141675/1‑54 (MQ=255)                                  gCGAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTtctc                            >  1:10762/1‑70 (MQ=255)                                    gAATCGCGAGGAAGACTTCCTTAATCTTACACATCa                                                            <  1:3162257/36‑1 (MQ=255)                                    gAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTTAGGAAGAAGTTTCTCGcc                         <  1:3105013/71‑1 (MQ=255)                                    gAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGcc                         <  1:3863930/71‑1 (MQ=255)                                    gAATCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGcc                         <  1:493514/71‑1 (MQ=255)                                     aaTCGCGAGGAAGACTTCCTTAATCTTACACATCAg                                                           <  1:694437/36‑1 (MQ=255)                                     aaTCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGc                          >  1:2962924/1‑69 (MQ=255)                                      aTCGCGAGGAAGACTTCCTTAATCTTACACATCAg                                                           <  1:2814778/35‑1 (MQ=255)                                      aTCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGa                                          >  1:3657750/1‑52 (MQ=255)                                      aTCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGa                                          >  1:2259103/1‑52 (MQ=255)                                      aTCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGt                                 <  1:1629950/61‑1 (MQ=255)                                      aTCGCGAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAg                       >  1:3186573/1‑71 (MQ=255)                                        cgcgAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGa                                          >  1:2612758/1‑50 (MQ=255)                                        cgcgAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGa                                          >  1:812394/1‑50 (MQ=255)                                         gcgAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAgct                     <  1:2766318/70‑1 (MQ=255)                                          cgAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAgctgc                   <  1:4138777/71‑1 (MQ=255)                                          cgAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAgctgc                   <  1:186089/71‑1 (MQ=255)                                           gAGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAgctgct                  <  1:3753485/71‑1 (MQ=255)                                            aGGAAGACTTCCTTAATCTTACACATCAgcgtgcgt                                                    <  1:1244394/36‑1 (MQ=255)                                            aGGAAGACTTCCTTAATCTTACACATCAgcgtgcgt                                                    <  1:506583/36‑1 (MQ=255)                                            aGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGgaagaa                                   <  1:3220528/53‑1 (MQ=255)                                            aGGAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGgaagaa                                   <  1:3649919/53‑1 (MQ=255)                                              gAAGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTggg               >  1:2847658/1‑71 (MQ=255)                                               aaGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTtc                              <  1:2928818/55‑1 (MQ=255)                                               aaGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGt              <  1:2965612/71‑1 (MQ=255)                                               aaGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGt              <  1:1053796/71‑1 (MQ=255)                                                aGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAgg                                        >  1:903430/1‑44 (MQ=255)                                                aGACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAgct                     >  1:1848753/1‑63 (MQ=255)                                                 gACTTCCTTAATCTTACACATCAGCGTGCGTTCTgg                                               <  1:2851049/36‑1 (MQ=255)                                                 gACTTCCTTAATCTTACACATCAGCGTGCGTTCTgg                                               <  1:1506884/36‑1 (MQ=255)                                                 gACTTCCTTAATCTTACACATCAGCGTGCGTTCTgg                                               <  1:141445/36‑1 (MQ=255)                                                 gACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTtctc                            >  1:3371528/1‑55 (MQ=255)                                                 gACTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTaa            <  1:36938/71‑1 (MQ=255)                                                   cTTCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATg          <  1:102952/71‑1 (MQ=255)                                                    ttCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGgaaga                                    <  1:35712/44‑1 (MQ=255)                                                    ttCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATgg         >  1:1219354/1‑71 (MQ=255)                                                    ttCCTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATgg         >  1:1473539/1‑71 (MQ=255)                                                      ccTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATGGTa       >  1:3285158/1‑71 (MQ=255)                                                       cTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATGGTAc      <  1:955758/71‑1 (MQ=255)                                                       cTTAATCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATGGTAc      <  1:132460/71‑1 (MQ=255)                                                        ttAATCTTACACATCAGCGTGCGTTCTGGACTGAGgaa                                      <  1:1008911/38‑1 (MQ=255)                                                          aaTCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTtctc                            >  1:1306041/1‑46 (MQ=255)                                                          aaTCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTaa            >  1:3267847/1‑62 (MQ=255)                                                          aaTCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATGGTACTTg   >  1:3911028/1‑71 (MQ=255)                                                           aTCTTACACATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATGGTACTTGc  <  1:2604690/71‑1 (MQ=255)                                                              ttACACATCAGCGTGCGTTCTGGACTGAGGaagaag                                  <  1:1596330/36‑1 (MQ=255)                                                                  |                                                                GAGGATCTCGTCTTTCGTCAGCAGCTGTTCAATGCGAATCGCGAGGAAGACTTCCTTAATCTTACGCATCAGCGTGCGTTCTGGACTGAGGAAGAAGTTTCTCGCCAGCTGCTGGGTAATGGTACTTGC  >  minE/2697746‑2697874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑