| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,706,320 | C→T | A95V (GCC→GTC) | rpe → | D‑ribulose‑5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,706,320 | 0 | C | T | 99.2% | 349.9 / NA | 117 | A95V (GCC→GTC) | rpe | D‑ribulose‑5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (50/66); minor base G (0/1); total (50/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 118 total aligned reads displayed.
ACCCGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGCCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTATT > minE/2706252‑2706386 | aCCCGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCt < 1:2573391/71‑1 (MQ=255) cccGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGtctc < 1:3157026/71‑1 (MQ=255) ccGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTcc < 1:293850/71‑1 (MQ=255) gTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTcc > 1:1702599/1‑69 (MQ=255) gTCGATCGCATTGTGCCTGATTTCGCTG‑CGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAg < 1:1609322/71‑1 (MQ=255) tCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAg > 1:3585209/1‑71 (MQ=255) tCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAg > 1:1616176/1‑71 (MQ=255) cGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAg > 1:1092328/1‑70 (MQ=255) gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGc < 1:2955245/70‑1 (MQ=255) gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGc < 1:1797386/70‑1 (MQ=255) gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa > 1:2179924/1‑71 (MQ=255) gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa < 1:3879219/71‑1 (MQ=255) gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa < 1:2332568/71‑1 (MQ=255) gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa < 1:2811872/71‑1 (MQ=255) tCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATg < 1:2947571/71‑1 (MQ=255) tCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATg > 1:904003/1‑71 (MQ=255) cGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGt > 1:1442230/1‑71 (MQ=255) cGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGt > 1:2036810/1‑71 (MQ=255) cGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGt > 1:3604225/1‑71 (MQ=255) gCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGtt < 1:3253389/71‑1 (MQ=255) gCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGtt < 1:2327132/71‑1 (MQ=255) cATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTg < 1:2345668/71‑1 (MQ=255) aTTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGa < 1:1363530/71‑1 (MQ=255) aTTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGa < 1:3013219/71‑1 (MQ=255) ttGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGAc < 1:3969923/71‑1 (MQ=255) ccTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGAcc > 1:699826/1‑67 (MQ=255) ccTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGAcc > 1:1398226/1‑67 (MQ=255) ccTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCAAGAAGTCTCCGAGCATGTTGa > 1:3621569/1‑65 (MQ=255) cTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGAGCGCACg < 1:5038/71‑1 (MQ=255) cTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACg > 1:1966094/1‑71 (MQ=255) cTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACg < 1:456489/71‑1 (MQ=255) tGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGc > 1:2694495/1‑71 (MQ=255) gATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa > 1:1116330/1‑56 (MQ=255) gATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa > 1:3062318/1‑56 (MQ=255) gATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa > 1:2765796/1‑56 (MQ=255) gATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCt > 1:3385460/1‑71 (MQ=255) tttCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGc < 1:1899882/71‑1 (MQ=255) gCTGCCGCTGGTGCCAGCATCATTAGCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt < 1:3007508/71‑1 (MQ=255) gCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAAc < 1:891350/70‑1 (MQ=255) gCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt < 1:1359238/71‑1 (MQ=255) cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCTCGCTGCAACTg < 1:132997/71‑1 (MQ=255) cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAAc < 1:507371/69‑1 (MQ=255) cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAAc < 1:696539/69‑1 (MQ=255) cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAAc < 1:3775602/69‑1 (MQ=255) cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTg < 1:833729/71‑1 (MQ=255) tGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCa < 1:205673/59‑1 (MQ=255) ccGCTGGTTCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGa < 1:2638785/69‑1 (MQ=255) ccGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCa > 1:956119/1‑57 (MQ=255) ccGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCa > 1:1321499/1‑57 (MQ=255) cGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTcc > 1:3758207/1‑40 (MQ=255) cGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa < 1:2872428/71‑1 (MQ=255) cGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa < 1:1790926/71‑1 (MQ=255) gCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt < 1:3419576/65‑1 (MQ=255) gCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa < 1:2683238/70‑1 (MQ=255) gCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa > 1:4141091/1‑70 (MQ=255) gCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa < 1:3181666/70‑1 (MQ=255) cTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa < 1:1145478/43‑1 (MQ=255) cTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGa > 1:2070338/1‑49 (MQ=255) tGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATg > 1:1115635/1‑44 (MQ=255) tGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa > 1:1780762/1‑68 (MQ=255) ggTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGtt > 1:3120597/1‑45 (MQ=255) ggTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGtt > 1:1423751/1‑45 (MQ=255) ccAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGaaaa < 1:1211020/70‑1 (MQ=255) ccAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGaaaa < 1:2242210/70‑1 (MQ=255) ccAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAAt < 1:1178372/71‑1 (MQ=255) ccAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAAt < 1:4136296/71‑1 (MQ=255) cAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt < 1:2155805/57‑1 (MQ=255) cAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt < 1:3394431/57‑1 (MQ=255) aGCATCATTACCTTTCATCCAGAAGTCTACGAGCATGTTGACCGCACGCTGCAACTGAt > 1:3499379/1‑59 (MQ=255) gCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGc > 1:1933762/1‑71 (MQ=255) gCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGc > 1:3855494/1‑71 (MQ=255) tcatTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGc < 1:1255717/45‑1 (MQ=255) tcatTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGc < 1:1562032/45‑1 (MQ=255) tcatTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTg < 1:184378/70‑1 (MQ=255) atTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa < 1:3257831/70‑1 (MQ=255) atTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa < 1:1457888/70‑1 (MQ=255) atTACCTTTCATCCAGAAGGCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa < 1:3683716/70‑1 (MQ=255) ttACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGa > 1:3240643/1‑58 (MQ=255) ttACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa > 1:771996/1‑69 (MQ=255) ttACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa > 1:2402526/1‑69 (MQ=255) aCCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAg < 1:3907479/70‑1 (MQ=255) ccTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGc < 1:328266/70‑1 (MQ=255) cTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGAt < 1:3840944/48‑1 (MQ=255) tttCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAg < 1:440624/52‑1 (MQ=255) ttCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGa > 1:3539895/1‑45 (MQ=255) ttCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa > 1:1259612/1‑48 (MQ=255) ttCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGaaaa < 1:3543855/55‑1 (MQ=255) tCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCggg < 1:3372425/69‑1 (MQ=255) tCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCggg < 1:3329170/69‑1 (MQ=255) tCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCggg < 1:315463/69‑1 (MQ=255) tCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTc > 1:2059963/1‑71 (MQ=255) aTCCAGAAGTCTCCGAGCATGTTGACCGCACGTTGCAACTGa > 1:3850983/1‑42 (MQ=38) aTCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCt < 1:2003460/70‑1 (MQ=255) aTCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTg < 1:1528830/71‑1 (MQ=255) tCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTgg < 1:1605515/71‑1 (MQ=255) cAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATgg > 1:2322127/1‑52 (MQ=255) cAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATgg > 1:1450395/1‑52 (MQ=255) cAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTa < 1:2897187/71‑1 (MQ=255) aGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTAt > 1:2131948/1‑71 (MQ=255) gAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTAtt > 1:153349/1‑71 (MQ=255) | ACCCGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGCCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTATT > minE/2706252‑2706386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |