Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,974,149	A→C	D182A (GAT→GCT)	nadR →	bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,974,149	0	A	C	100.0%	267.4 / NA	71	D182A (GAT→GCT)	nadR	bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (44/27);  total (44/27)

CGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTAT  >  minE/2974081‑2974215                                                                     |                                                                   cGGCATCAAAAAGTTTATGGCTTAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTg                                                                  <  1:1621204/71‑1 (MQ=255)       cAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCca                                                             >  1:3011995/1‑70 (MQ=255)        aaaaaGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCcaca                                                           <  1:2686247/71‑1 (MQ=255)         aaaaGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCcac                                                            >  1:2359366/1‑69 (MQ=255)          aaaGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAg                                                          >  1:2721057/1‑70 (MQ=255)          aaaGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGt                                                         <  1:2908677/71‑1 (MQ=255)                tATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATg                                                    >  1:4096867/1‑70 (MQ=255)                tATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATg                                                    <  1:2141550/70‑1 (MQ=255)                tATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGAGCCACAGTATATg                                                    <  1:348475/70‑1 (MQ=255)                  tGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGaa                                                 >  1:3824619/1‑71 (MQ=255)                    gCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAAc                                                <  1:2771685/70‑1 (MQ=255)                    gCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACa                                               >  1:2257139/1‑71 (MQ=255)                      tGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATc                                             >  1:2513287/1‑71 (MQ=255)                      tGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATc                                             >  1:2148923/1‑71 (MQ=255)                          aaaGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTggg                                         <  1:2270382/71‑1 (MQ=255)                           aaGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTgggg                                        <  1:3094062/71‑1 (MQ=255)                               gATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATc                                     >  1:3870754/1‑70 (MQ=255)                                aTCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATc                                     >  1:2303829/1‑69 (MQ=255)                                 tCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCga                                   <  1:1826241/70‑1 (MQ=255)                                  ccAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCgaga                                 >  1:2548314/1‑71 (MQ=255)                                   cAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCgaga                                 >  1:2534996/1‑70 (MQ=255)                                   cAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCgaga                                 <  1:1858965/70‑1 (MQ=255)                                   cAGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTCGGGATCgaga                                 >  1:2953732/1‑70 (MQ=255)                                    aGCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACg                               >  1:2203826/1‑71 (MQ=255)                                     gCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACgg                              >  1:293091/1‑71 (MQ=255)                                     gCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACgg                              >  1:2526665/1‑71 (MQ=255)                                     gCCGGATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACgg                              >  1:504329/1‑71 (MQ=255)                                      ccGGATCTGATCTACACCTCGGAAGAAGCCGCTgcgc                                                               <  1:1868913/37‑1 (MQ=255)                                        ggATCTGATCTACACCTCGGAAGAAGCCGCTGCGCCACAGtat                                                       >  1:1625589/1‑43 (MQ=255)                                         gatctgatctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCt                          >  1:2844165/1‑71 (MQ=255)                                            ctgatctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCga                                   <  1:3463312/59‑1 (MQ=255)                                            ctgatctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGt                       >  1:4035964/1‑71 (MQ=255)                                             tgatctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGATgg                        >  1:2764769/1‑69 (MQ=255)                                              gatctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTc                      >  1:4038029/1‑70 (MQ=255)                                               atctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATg                                                    >  1:313131/1‑39 (MQ=255)                                               atctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTg                         <  1:2438506/66‑1 (MQ=255)                                               atctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTc                      >  1:3993380/1‑69 (MQ=255)                                               atctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCg                     <  1:1916376/70‑1 (MQ=255)                                               atctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGa                    >  1:2355433/1‑71 (MQ=255)                                               atctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGa                    >  1:1099892/1‑71 (MQ=255)                                                tctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCgaga                                 >  1:1643584/1‑57 (MQ=255)                                                tctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCgaga                                 >  1:147102/1‑57 (MQ=255)                                                tctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCgaga                                 >  1:1049146/1‑57 (MQ=255)                                                tctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGAt                   >  1:2461420/1‑71 (MQ=255)                                                 ctACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATc                  >  1:96788/1‑71 (MQ=255)                                                  tACACCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATcc                 >  1:2286380/1‑71 (MQ=255)                                                    cacCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGa                    <  1:2028340/66‑1 (MQ=255)                                                    cacCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGAt                   <  1:3022902/67‑1 (MQ=255)                                                    cacCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGAt                   <  1:3521369/67‑1 (MQ=255)                                                    cacCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGAt                   <  1:3559975/67‑1 (MQ=255)                                                    cacCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATcc                 <  1:796326/69‑1 (MQ=255)                                                    cacCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGa               <  1:3344983/71‑1 (MQ=255)                                                     acCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGAt                   <  1:135263/66‑1 (MQ=255)                                                     acCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGaa              <  1:4022676/71‑1 (MQ=255)                                                     acCTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGCCGATCCGaa              <  1:541412/71‑1 (MQ=255)                                                      ccTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGaaa             >  1:2871801/1‑71 (MQ=255)                                                      ccTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGaaa             <  1:3871938/71‑1 (MQ=255)                                                       cTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAAc            >  1:1071317/1‑71 (MQ=255)                                                       cTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAAc            >  1:1197543/1‑71 (MQ=255)                                                       cTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAAc            <  1:1906128/71‑1 (MQ=255)                                                       cTCGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAAc            >  1:2825990/1‑71 (MQ=255)                                                         cGGAAGAAGCCGCTGCGCCACAGTATATGGAACATCTg                                           >  1:621585/1‑38 (MQ=255)                                                            aagaagCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTAc        >  1:3600928/1‑70 (MQ=255)                                                               aagCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGaa              <  1:3399748/61‑1 (MQ=255)                                                                agCCGCTGCGCCACAGTATATGGAACATCTGGGGAt                                      >  1:2389011/1‑36 (MQ=255)                                                                agCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTAc        >  1:312157/1‑66 (MQ=255)                                                                agCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTa   >  1:3888860/1‑71 (MQ=255)                                                                agCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTa   >  1:2406030/1‑71 (MQ=255)                                                                agCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTa   >  1:3534316/1‑71 (MQ=255)                                                                 gCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCg                     >  1:479050/1‑52 (MQ=255)                                                                 gCCGCTGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTAt  <  1:662936/71‑1 (MQ=255)                                                                     |                                                                   CGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTAT  >  minE/2974081‑2974215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑