BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	171,024	C→T	D115D (GAC→GAT)	pyrH →	uridylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	171,024	0	C	T	100.0%	294.5 / NA	94	D115D (GAC→GAT)	pyrH	uridylate kinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (37/57); total (37/57)

Only 100 of 110 total aligned reads displayed.

ATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGT  >  minE/170957‑171090

aTGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagc                                                                 >  1:1668606/1‑71 (MQ=255)

  gcactgcacCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagcta                                                               >  1:3957966/1‑71 (MQ=255)

   cactgcacCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagcta                                                               <  1:2821952/70‑1 (MQ=255)

   cactgcacCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagcta                                                               <  1:3220638/70‑1 (MQ=255)

    actgcacCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagctaca                                                             <  1:1876720/71‑1 (MQ=255)

     ctgcacCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagctacag                                                            >  1:261136/1‑71 (MQ=255)

     ctgcacCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagctacag                                                            >  1:152321/1‑71 (MQ=255)

      tgcacCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagctacag                                                            >  1:3389883/1‑70 (MQ=255)

        cacCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagctacagct                                                          <  1:3963798/70‑1 (MQ=255)

         acCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagctacagc                                                           <  1:2568660/68‑1 (MQ=255)

         acCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTgg                                                        <  1:3982272/71‑1 (MQ=255)

         acCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTgg                                                        <  1:3992471/71‑1 (MQ=255)

         acCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTgg                                                        >  1:4079556/1‑71 (MQ=255)

         acCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTgg                                                        <  1:2247751/71‑1 (MQ=255)

          ccGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTggg                                                       <  1:649945/71‑1 (MQ=255)

          ccGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTggg                                                       <  1:949381/71‑1 (MQ=255)

             cgcCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTggg                                                       >  1:2994076/1‑68 (MQ=255)

             cgcCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAg                                                    <  1:471268/71‑1 (MQ=255)

              gcCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGa                                                   <  1:3207509/71‑1 (MQ=255)

               ccTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAg                                                    <  1:347384/69‑1 (MQ=255)

                cTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAg                                                 <  1:1231012/71‑1 (MQ=255)

                cTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAg                                                 >  1:813787/1‑71 (MQ=255)

                cTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAg                                                 <  1:1287797/71‑1 (MQ=255)

                  aTGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGc                                                >  1:174625/1‑70 (MQ=255)

                   tgtgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagcta                                                               <  1:1447244/54‑1 (MQ=255)

                   tgtgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGc                                                <  1:2530166/69‑1 (MQ=255)

                   tgtgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTa                                              <  1:3350419/71‑1 (MQ=255)

                   tgtgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTa                                              <  1:2884122/71‑1 (MQ=255)

                   tgtgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTa                                              <  1:3355105/71‑1 (MQ=255)

                   tgtgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTa                                              <  1:1794317/71‑1 (MQ=255)

                    gtgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagctacagct                                                          <  1:2144030/58‑1 (MQ=255)

                    gtgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGatagctacagct                                                          <  1:126570/58‑1 (MQ=255)

                    gtgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTa                                              <  1:4146626/70‑1 (MQ=255)

                     tgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTAt                                             <  1:3307399/70‑1 (MQ=255)

                     tgAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATc                                            <  1:1406498/71‑1 (MQ=255)

                      gAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTAt                                             >  1:2302808/1‑69 (MQ=255)

                      gAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTAt                                             >  1:2906169/1‑69 (MQ=255)

                        aCGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGc                                         >  1:551549/1‑71 (MQ=255)

                         cGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGcc                                        >  1:129459/1‑71 (MQ=255)

                         cGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGcc                                        >  1:712651/1‑71 (MQ=255)

                          gCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCt                                       >  1:3578504/1‑71 (MQ=255)

                           cTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTg                                      <  1:3943987/71‑1 (MQ=255)

                           cTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTg                                      <  1:3649097/71‑1 (MQ=255)

                           cTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTg                                      <  1:3223239/71‑1 (MQ=255)

                           cTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTg                                      <  1:607103/71‑1 (MQ=255)

                            tCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTg                                      <  1:1812697/70‑1 (MQ=255)

                            tCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTg                                      <  1:1362604/70‑1 (MQ=255)

                             cGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGtt                                    >  1:3399649/1‑71 (MQ=255)

                              gTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTAt                                             >  1:2262228/1‑61 (MQ=255)

                              gTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTg                                   >  1:4045174/1‑71 (MQ=255)

                                 tGATGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTgcg                                 >  1:3743591/1‑70 (MQ=255)

                                   aTGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTgcgc                                <  1:190050/69‑1 (MQ=255)

                                   aTGTCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGcaa                              <  1:1898568/71‑1 (MQ=255)

                                      tCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGcaacaa                           >  1:1714546/1‑71 (MQ=255)

                                      tCCGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGcaaca                            <  1:3913621/70‑1 (MQ=255)

                                        cGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTgcgc                                >  1:2646945/1‑64 (MQ=255)

                                        cGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAAcc                         <  1:3748637/71‑1 (MQ=255)

                                        cGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAAcc                         <  1:360993/71‑1 (MQ=255)

                                        cGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAAcc                         <  1:4058367/71‑1 (MQ=255)

                                        cGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAAcc                         <  1:1312660/71‑1 (MQ=255)

                                        cGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAAcc                         <  1:3327295/71‑1 (MQ=255)

                                        cGCTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAAcc                         <  1:2887043/71‑1 (MQ=255)

                                          cTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTgg                                                        >  1:569438/1‑38 (MQ=255)

                                          cTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgt                       >  1:1565601/1‑71 (MQ=255)

                                          cTATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgt                       <  1:2917922/71‑1 (MQ=255)

                                           tATTCCATTGAATGGCGTGTGCGatagctacagct                                                          <  1:942920/35‑1 (MQ=255)

                                           tATTCCATTGAATGGCGTGTGCGatagctacagct                                                          <  1:3850036/35‑1 (MQ=255)

                                           tATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgtg                      >  1:1280394/1‑71 (MQ=255)

                                           tATTCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgt                       >  1:1285139/1‑70 (MQ=255)

                                             ttCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgtg                      <  1:134680/69‑1 (MQ=255)

                                             ttCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgtg                      <  1:3813915/69‑1 (MQ=255)

                                             ttCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgtg                      <  1:1911538/69‑1 (MQ=255)

                                             ttCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgtg                      <  1:3666192/69‑1 (MQ=255)

                                             ttCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgtg                      <  1:2732823/69‑1 (MQ=255)

                                             ttCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCgtg                      <  1:1380644/69‑1 (MQ=255)

                                              tCCATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCt                                               <  1:3545805/43‑1 (MQ=255)

                                               ccATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAg                                          >  1:3625016/1‑47 (MQ=255)

                                               ccATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAg                                          >  1:812478/1‑47 (MQ=255)

                                               ccATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAg                                          >  1:1048047/1‑47 (MQ=255)

                                                cATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTgtggt                  <  1:2640076/70‑1 (MQ=255)

                                                cATTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTgtggt                  <  1:2441577/70‑1 (MQ=255)

                                                 aTTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGa                <  1:2178851/71‑1 (MQ=255)

                                                 aTTGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGa                <  1:262378/71‑1 (MQ=255)

                                                  ttGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGAt               >  1:2106422/1‑71 (MQ=255)

                                                  ttGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGAt               >  1:502160/1‑71 (MQ=255)

                                                  ttGAATGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGAt               >  1:1252759/1‑71 (MQ=255)

                                                     aaTGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTTATCct            >  1:635538/1‑71 (MQ=255)

                                                     aaTGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCct            >  1:1950312/1‑71 (MQ=255)

                                                      aTGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCctc           <  1:3576530/71‑1 (MQ=255)

                                                       tGGCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCctct          >  1:3517307/1‑71 (MQ=255)

                                                         gCGTGTGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTcc        >  1:402928/1‑71 (MQ=255)

                                                           gtgtGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTgtggt                  >  1:787873/1‑59 (MQ=255)

                                                           gtgtGCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTccgc      <  1:1853336/71‑1 (MQ=255)

                                                            tgtgCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTccgc      <  1:3476533/70‑1 (MQ=255)

                                                             gtgCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTacgccg    >  1:3154626/1‑71 (MQ=255)

                                                              tgCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCgg   >  1:2540306/1‑71 (MQ=255)

                                                               gCGATAGCTACAGCTTGGCAGAAGCTATCAGCCTg                                      <  1:1439710/35‑1 (MQ=37)

                                                               gCGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTccgccg    >  1:711571/1‑69 (MQ=255)

                                                                cGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATc              <  1:1453849/58‑1 (MQ=255)

                                                                cGATAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGt  <  1:762983/70‑1 (MQ=255)

ATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGT  >  minE/170957‑171090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`