Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	204,511	C→T	intergenic (+111/‑73)	alaV → / → rrlH	tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	204,511	0	C	T	100.0%	33.5 / NA	13	intergenic (+111/‑73)	alaV/rrlH	tRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/4);  total (9/4)

AATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGG  >  minE/204493‑204577                   |                                                                   aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa                <  1:4020698/71‑1 (MQ=255)    tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa              >  1:1495154/1‑70 (MQ=255)      aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa            >  1:2698224/1‑70 (MQ=255)       aaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa          >  1:1352974/1‑71 (MQ=255)       aaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa          <  1:1438854/71‑1 (MQ=255)        acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa                >  1:3008423/1‑64 (MQ=255)        acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt         >  1:1409159/1‑71 (MQ=255)        acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt         >  1:885818/1‑71 (MQ=255)           cTGAACAATGAAAGTAGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt      <  1:1928486/71‑1 (MQ=11)            tGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa                <  1:67852/60‑1 (MQ=11)            tGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc     >  1:2217494/1‑71 (MQ=255)             gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc           >  1:966032/1‑64 (MQ=255)             gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg    >  1:4116448/1‑71 (MQ=255)               acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg  >  1:3527183/1‑71 (MQ=255)                caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg  >  1:2801368/1‑70 (MQ=255)                   |                                                                   AATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGG  >  minE/204493‑204577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑