BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	204,556	A→G	intergenic (+156/‑28)	alaV → / → rrlH	tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	204,556	0	A	G	100.0%	206.3 / NA	65	intergenic (+156/‑28)	alaV/rrlH	tRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/34); total (31/34)

AATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGG  >  minE/204493‑204619

aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa                                                          <  1:4020698/71‑1 (MQ=255)

   tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                                                        >  1:1495154/1‑70 (MQ=255)

     aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa                                                      >  1:2698224/1‑70 (MQ=255)

      aaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa                                                    >  1:1352974/1‑71 (MQ=255)

      aaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa                                                    <  1:1438854/71‑1 (MQ=255)

       acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa                                                          >  1:3008423/1‑64 (MQ=255)

       acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt                                                   >  1:1409159/1‑71 (MQ=255)

       acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt                                                   >  1:885818/1‑71 (MQ=255)

          cTGAACAATGAAAGTAGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt                                                <  1:1928486/71‑1 (MQ=11)

           tGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa                                                          <  1:67852/60‑1 (MQ=11)

           tGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc                                               >  1:2217494/1‑71 (MQ=255)

            gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                     >  1:966032/1‑64 (MQ=255)

            gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg                                              >  1:4116448/1‑71 (MQ=255)

              acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg                                            >  1:3527183/1‑71 (MQ=255)

               caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg                                            >  1:2801368/1‑70 (MQ=255)

                 atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGtt                                          <  1:847884/68‑1 (MQ=255)

                  tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                        >  1:3064473/2‑71 (MQ=19)

                  tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                        >  1:2180376/2‑71 (MQ=19)

                    aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                       <  1:3574412/70‑1 (MQ=25)

                    aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                        >  1:471085/1‑69 (MQ=25)

                      aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                     >  1:1951651/1‑70 (MQ=25)

                        ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                  <  1:3383298/71‑1 (MQ=21)

                          gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                       <  1:3009443/64‑1 (MQ=25)

                             cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                              <  1:3482054/70‑1 (MQ=25)

                             cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                              <  1:1724168/70‑1 (MQ=25)

                             cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                              <  1:1693268/70‑1 (MQ=25)

                             cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                              >  1:1658292/1‑70 (MQ=25)

                              gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg                                              <  1:357888/53‑1 (MQ=255)

                              gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                       <  1:3231466/60‑1 (MQ=255)

                               tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                       <  1:11940/59‑1 (MQ=255)

                                 aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa                                      <  1:2396827/58‑1 (MQ=255)

                                 aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa                                      <  1:1197524/58‑1 (MQ=255)

                                 aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc                           <  1:4092621/69‑1 (MQ=25)

                                 aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                          <  1:1629103/70‑1 (MQ=25)

                                  gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                              >  1:2048485/1‑65 (MQ=255)

                                  gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                          >  1:2190177/1‑69 (MQ=25)

                                  gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTaa                        <  1:1915933/71‑1 (MQ=21)

                                   tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                       <  1:651855/55‑1 (MQ=255)

                                    ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                     <  1:1060374/56‑1 (MQ=255)

                                     tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                     <  1:1185761/39‑1 (MQ=255)

                                       tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                     <  1:1165843/69‑1 (MQ=25)

                                       tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                     <  1:1749985/69‑1 (MQ=25)

                                          aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg                                             >  1:709495/1‑42 (MQ=255)

                                          aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                <  1:3426624/71‑1 (MQ=21)

                                          aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                <  1:2820916/71‑1 (MQ=21)

                                           aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg               >  1:496280/1‑71 (MQ=21)

                                           aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg               <  1:2324545/71‑1 (MQ=21)

                                                cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTgg           <  1:1661032/70‑1 (MQ=25)

                                                cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa          >  1:3608758/1‑71 (MQ=21)

                                                 gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGAt         <  1:519112/71‑1 (MQ=21)

                                                   aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGTTTAAGCGACTAAGCGTACACGGTGGATg        <  1:4147027/70‑1 (MQ=11)

                                                   aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc       >  1:1862807/1‑71 (MQ=21)

                                                   aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc       >  1:3440507/1‑71 (MQ=21)

                                                    acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                <  1:3650029/61‑1 (MQ=25)

                                                     cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                  >  1:3999286/1‑42 (MQ=255)

                                                     cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc     >  1:1463358/1‑71 (MQ=21)

                                                     cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc      >  1:901375/1‑70 (MQ=25)

                                                       cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg            >  1:2854724/1‑62 (MQ=25)

                                                       cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg   >  1:998077/1‑71 (MQ=21)

                                                        gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                          <  1:2239045/47‑1 (MQ=255)

                                                        gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                          <  1:2117023/47‑1 (MQ=255)

                                                        gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt    <  1:2878766/69‑1 (MQ=25)

                                                         atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  >  1:3063438/1‑70 (MQ=25)

                                                         atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  >  1:3058603/1‑70 (MQ=25)

                                                         atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  >  1:223445/1‑70 (MQ=25)

                                                          tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  >  1:502106/1‑69 (MQ=25)

                                                          tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  >  1:2308437/1‑69 (MQ=25)

                                                          tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgc  >  1:2618138/1‑68 (MQ=255)

                                                            atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                          <  1:3282652/43‑1 (MQ=255)

                                                            atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                     <  1:3054067/48‑1 (MQ=255)

AATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGG  >  minE/204493‑204619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`