BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	269,873	G→A	intergenic (‑61/‑145)	xseB ← / → thiI	exonuclease VII small subunit/sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	269,873	0	G	A	100.0%	295.9 / NA	95	intergenic (‑61/‑145)	xseB/thiI	exonuclease VII small subunit/sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (50/45); total (50/45)

Only 100 of 118 total aligned reads displayed.

CGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAGCAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTG  >  minE/269807‑269940

cGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGgcaaataacaaa                                                                 >  1:1577350/1‑71 (MQ=255)

cGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGgcaaataaca                                                                   <  1:2216286/69‑1 (MQ=255)

 ggCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGgcaaataacaaa                                                                 >  1:3311687/1‑70 (MQ=255)

      aatgaatgTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAAcgc                                                           >  1:382129/1‑71 (MQ=255)

       atgaatgTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGgcaaataacaaat                                                                >  1:1241758/1‑65 (MQ=255)

       atgaatgTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAAcgcg                                                          >  1:1051030/1‑71 (MQ=255)

        tgaatgTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAAcgcg                                                          >  1:1406505/1‑70 (MQ=255)

        tgaatgTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACgcgc                                                         <  1:4118431/71‑1 (MQ=255)

          aatgTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCaa                                                       <  1:1566311/71‑1 (MQ=255)

          aatgTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCa                                                        <  1:3962578/70‑1 (MQ=255)

          aatgTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCa                                                        <  1:204690/70‑1 (MQ=255)

             gTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGgcaaataacaaata                                                               <  1:2931314/60‑1 (MQ=255)

              tCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGt                                                   <  1:314888/71‑1 (MQ=255)

               cTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCa                                                        <  1:3802178/65‑1 (MQ=255)

               cTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCa                                                        <  1:3728235/65‑1 (MQ=255)

                  aCTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTa                                                  <  1:3085600/68‑1 (MQ=255)

                  aCTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTa                                                  <  1:3080633/68‑1 (MQ=255)

                  aCTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTa                                                  <  1:3342161/68‑1 (MQ=255)

                  aCTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAgg                                               >  1:1150165/1‑71 (MQ=255)

                  aCTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAg                                                <  1:355371/70‑1 (MQ=255)

                  aCTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAg                                                <  1:3370081/70‑1 (MQ=255)

                   ctctCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAgg                                               >  1:2786413/1‑70 (MQ=255)

                   ctctCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGt                                              <  1:1554501/71‑1 (MQ=255)

                   ctctCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGt                                              >  1:717235/1‑71 (MQ=255)

                   ctctCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGt                                              >  1:2037982/1‑71 (MQ=255)

                      tcAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTg                                             <  1:2875615/69‑1 (MQ=255)

                        aaTATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGAtgt                                         >  1:3484289/1‑71 (MQ=255)

                         atatTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGAtgtg                                        >  1:940981/1‑71 (MQ=255)

                         atatTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGAtgtg                                        <  1:1297288/71‑1 (MQ=255)

                         atatTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGAtgtg                                        >  1:380804/1‑71 (MQ=255)

                         atatTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGAtgt                                         >  1:3855206/1‑70 (MQ=255)

                           atTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAAcgc                                                           >  1:3701758/1‑50 (MQ=255)

                            tttttCGCCCCGTCATGGTAACGGACTCAGGGCaaataacaaataac                                                             >  1:585857/1‑47 (MQ=255)

                            tttttCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGa                                            <  1:2201823/64‑1 (MQ=255)

                            tttttCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGca                                      >  1:2432050/1‑70 (MQ=255)

                            tttttCGCCCCGTCATGGTAACGGACTAAGGGCAAATAACAAATAAcg                                                            >  1:79703/1‑48 (MQ=255)

                               ttCGCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAg                                   >  1:3727900/1‑70 (MQ=255)

                                  gCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATg                                                     <  1:2988981/49‑1 (MQ=255)

                                  gCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATg                                                     <  1:2125160/49‑1 (MQ=255)

                                  gCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATg                                                     <  1:2741064/49‑1 (MQ=255)

                                  gCCCCGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATg                                                     <  1:2859939/49‑1 (MQ=255)

                                   ccccGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAg                              >  1:814480/1‑71 (MQ=255)

                                    cccGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTg                                             <  1:2749905/55‑1 (MQ=255)

                                     ccGTCATGGTAACGGACTCAGGgcaaataacaaat                                                                >  1:2630371/1‑35 (MQ=255)

                                     ccGTCATGGTAACGGACTCAGGgcaaataacaaat                                                                <  1:2147172/35‑1 (MQ=255)

                                     ccGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAAt                                                      >  1:860062/1‑45 (MQ=255)

                                     ccGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAgg                                               <  1:4141901/52‑1 (MQ=255)

                                     ccGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAgg                                               >  1:565902/1‑52 (MQ=255)

                                     ccGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGc                             >  1:494702/1‑70 (MQ=255)

                                     ccGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGc                             >  1:3215187/1‑70 (MQ=255)

                                     ccGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGc                             >  1:618910/1‑70 (MQ=255)

                                     ccGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGAGCAAt                                                      >  1:1373240/1‑45 (MQ=255)

                                      cGTCATGGTAACGGACTCAGGGCAAATAACAAATAACTCGCAATGGTAAGGTGATGTGCACAGCAAAGCGa                           >  1:3638188/1‑71 (MQ=255)

                                      cGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCaa                                <  1:156062/66‑1 (MQ=255)

                                      cGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGa                           <  1:2234558/71‑1 (MQ=255)

                                      cGTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGa                           >  1:1996338/1‑71 (MQ=255)

                                      cGTCATAGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGa                           >  1:1632/1‑71 (MQ=255)

                                       gTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGt                                                   <  1:3545532/46‑1 (MQ=255)

                                       gTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGAt                          <  1:1135475/71‑1 (MQ=255)

                                       gTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGAt                          <  1:1967834/71‑1 (MQ=255)

                                       gTCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGAt                          <  1:2264464/71‑1 (MQ=255)

                                        tCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATg                         <  1:887832/71‑1 (MQ=255)

                                        tCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATg                         <  1:3759283/71‑1 (MQ=255)

                                        tCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATg                         >  1:3450847/1‑71 (MQ=255)

                                        tCATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATg                         <  1:3910961/71‑1 (MQ=255)

                                         cATGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAg                                                >  1:1205571/1‑47 (MQ=255)

                                          aTGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTaa                                                 >  1:76795/1‑45 (MQ=255)

                                          aTGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTaa                                                 >  1:4093959/1‑45 (MQ=255)

                                          aTGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTaa                                                 >  1:2475157/1‑45 (MQ=255)

                                          aTGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCaa                                <  1:4083896/62‑1 (MQ=255)

                                          aTGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGtt                       <  1:3250759/71‑1 (MQ=255)

                                          aTGGTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGt                        <  1:728284/70‑1 (MQ=255)

                                            ggTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAg                     >  1:512139/1‑71 (MQ=255)

                                            ggTAACGGACTCAGGGCAAATAACAAATAACGAGCAATGGTAAGGTGAtg                                          >  1:3664543/1‑50 (MQ=255)

                                             gTAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAg                     <  1:2272394/70‑1 (MQ=255)

                                              tAACGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTg                   <  1:2741856/71‑1 (MQ=255)

                                                aCGGACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTgg                  >  1:2461551/1‑70 (MQ=255)

                                                  ggACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGAt                          >  1:1877566/1‑60 (MQ=255)

                                                   gACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCaa                                >  1:3058332/1‑53 (MQ=255)

                                                   gACTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGt                 <  1:1940916/68‑1 (MQ=255)

                                                     cTCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACt            <  1:3243302/71‑1 (MQ=255)

                                                      tCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAg                     <  1:664474/61‑1 (MQ=255)

                                                      tCAGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGta                <  1:893170/66‑1 (MQ=255)

                                                        aGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTg                   >  1:1642178/1‑61 (MQ=255)

                                                        aGGGCAAATAACAAATAACGCGCAATGGTAAGGTGATGTCCACACCaaa                               >  1:54401/1‑49 (MQ=255)

                                                         gggCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCcg        >  1:899330/1‑71 (MQ=255)

                                                         gggCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCcg        >  1:4119090/1‑71 (MQ=255)

                                                          ggCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCcgc       >  1:695846/1‑71 (MQ=255)

                                                          ggCAAATAACAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCcgc       >  1:3697916/1‑71 (MQ=255)

                                                           gcaaataacaaataACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCcgcg      >  1:526726/1‑71 (MQ=255)

                                                            caaataacaaataaCGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCgcgc     >  1:1660654/1‑71 (MQ=255)

                                                             aaataacaaataacGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACtt           <  1:1587987/64‑1 (MQ=255)

                                                             aaataacaaataacGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTcc         <  1:2722417/66‑1 (MQ=255)

                                                             aaataacaaataacGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCgcgc     <  1:572477/70‑1 (MQ=255)

                                                             aaataacaaataacGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGcc    >  1:520906/1‑71 (MQ=255)

                                                              aataacaaataACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGt                    <  1:616678/54‑1 (MQ=255)

                                                              aataacaaataACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCt   <  1:2550360/71‑1 (MQ=255)

                                                              aataacaaataACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCt   <  1:2847895/71‑1 (MQ=255)

                                                              aataacaaataACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCt   <  1:3351043/71‑1 (MQ=255)

                                                               ataacaaataACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTg  >  1:1533740/1‑71 (MQ=255)

CGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAGCAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTG  >  minE/269807‑269940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`