BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	364,085	C→T	G15D (GGC→GAC)	intD ←	predicted integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	364,085	0	C	T	100.0%	303.4 / NA	97	G15D (GGC→GAC)	intD	predicted integrase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (45/52); total (45/52)

Only 100 of 102 total aligned reads displayed.

CCATTTCTATGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGCCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGACAGCCT  >  minE/364018‑364151

ccATTTCTATGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAAtcagtcag                                                                 >  1:1007873/1‑71 (MQ=255)

ccATTTCTATGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAAtcagtcag                                                                 <  1:3875570/71‑1 (MQ=255)

 cATTTCTATGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGTGACGCCTGACTGAATcagtcagc                                                                <  1:3137976/71‑1 (MQ=255)

   tttCTATGGACTCCCATCCCCCCATTTCCTGAAGCACAGATAATGGGACGCCTGACTGAATCAGTCAGCtt                                                              >  1:1903957/1‑71 (MQ=255)

         tGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGccc                                                          <  1:473665/69‑1 (MQ=255)

         tGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGccc                                                          <  1:3877358/69‑1 (MQ=255)

         tGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAg                                                        <  1:2281817/71‑1 (MQ=255)

         tGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAg                                                        <  1:3892486/71‑1 (MQ=255)

         tGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAg                                                        <  1:1469269/71‑1 (MQ=255)

         tGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAg                                                        <  1:3540935/71‑1 (MQ=255)

          ggAGTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAg                                                        >  1:1883769/1‑70 (MQ=255)

          ggACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAgg                                                       >  1:1228597/1‑71 (MQ=255)

          ggACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAgg                                                       >  1:3019880/1‑71 (MQ=255)

          ggACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAg                                                        >  1:3071354/1‑70 (MQ=255)

           gACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGgt                                                      <  1:418569/71‑1 (MQ=255)

           gACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGgt                                                      <  1:2806597/71‑1 (MQ=255)

             cTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGgtgt                                                    <  1:2856390/71‑1 (MQ=255)

                ccATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTc                                                 >  1:2953165/1‑71 (MQ=255)

                ccATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTc                                                 >  1:2618127/1‑71 (MQ=255)

                ccATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTc                                                 >  1:503136/1‑71 (MQ=255)

                ccATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTT‑CCCAGGTGTGTCt                                                >  1:1520083/1‑71 (MQ=255)

                 cATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCt                                                <  1:373147/71‑1 (MQ=255)

                  atacGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCt                                                >  1:15236/4‑70 (MQ=255)

                  aTCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCa                                                         <  1:195872/61‑1 (MQ=255)

                  aTCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCa                                                         <  1:2200873/61‑1 (MQ=255)

                  aTCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCa                                                         <  1:1073588/61‑1 (MQ=255)

                  aTCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTg                                               <  1:3222450/71‑1 (MQ=255)

                  aTCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTg                                               <  1:908717/71‑1 (MQ=255)

                   tCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGgtgtgt                                                  <  1:2419132/67‑1 (MQ=255)

                   tCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGgtgt                                                    <  1:2125957/65‑1 (MQ=255)

                     cGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAgg                                            >  1:990291/1‑71 (MQ=255)

                     cGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAgg                                            >  1:2098394/1‑71 (MQ=255)

                      gCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGgtgt                                                    <  1:973481/62‑1 (MQ=255)

                        ccATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCa                                         >  1:2760936/1‑71 (MQ=255)

                        ccATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCa                                         <  1:2392474/71‑1 (MQ=255)

                         cATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGcca                                                          >  1:2601821/1‑52 (MQ=255)

                         cATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGgt                                                      >  1:452387/1‑57 (MQ=255)

                          aTTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGgt                                                      >  1:2319886/1‑56 (MQ=255)

                          aTTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATg                                       >  1:3313766/1‑71 (MQ=255)

                          aTTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATg                                       >  1:1195609/1‑71 (MQ=255)

                           tttCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATg                                       >  1:1514534/1‑70 (MQ=255)

                             tCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCGGCTTGCCCAGGTGTGTCTGAGGTCATGGa                                     >  1:810850/1‑70 (MQ=255)

                             tCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCAt                                        >  1:320761/1‑67 (MQ=255)

                              ccTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGaaa                                   >  1:883334/1‑71 (MQ=255)

                              ccTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGaa                                    <  1:1499054/70‑1 (MQ=255)

                              ccTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGaa                                    <  1:3786690/70‑1 (MQ=255)

                              ccTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGaa                                    <  1:1844946/70‑1 (MQ=255)

                               cTGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGaaa                                   <  1:1951055/70‑1 (MQ=255)

                                tGAAGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACg                                 >  1:2334247/1‑71 (MQ=255)

                                  aaGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAAc                                  >  1:1656166/1‑68 (MQ=255)

                                   aGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATgg                                      >  1:2418526/1‑63 (MQ=255)

                                   aGCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGa                               >  1:3519487/1‑70 (MQ=255)

                                    gCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGaaa                             >  1:1941494/1‑71 (MQ=255)

                                    gCACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGaaa                             <  1:366128/71‑1 (MQ=255)

                                     cACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCt                                                <  1:224387/51‑1 (MQ=255)

                                     cACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAAt                            <  1:2237175/71‑1 (MQ=255)

                                     cACTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAAt                            <  1:3845790/71‑1 (MQ=255)

                                      aCTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATgg                                      <  1:131025/60‑1 (MQ=255)

                                       cTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCt                          <  1:407337/71‑1 (MQ=255)

                                       cTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCt                          <  1:3204152/71‑1 (MQ=255)

                                       cTGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCt                          <  1:235816/71‑1 (MQ=255)

                                        tGATAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCtt                         >  1:1486835/1‑71 (MQ=255)

                                          aTAATGGGACGCGTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCttt                        <  1:3269394/70‑1 (MQ=255)

                                          aTAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCttt                        >  1:1511905/1‑70 (MQ=255)

                                          aTAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCttt                        >  1:3409981/1‑70 (MQ=255)

                                           tAATGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGaa                                    <  1:2654484/57‑1 (MQ=255)

                                            aaTGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCttt                        >  1:3161470/1‑68 (MQ=255)

                                            aaTGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCttt                        >  1:1207971/1‑68 (MQ=255)

                                             aTGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAAt                            <  1:77906/63‑1 (MQ=255)

                                             aTGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAAt                            <  1:2487876/63‑1 (MQ=255)

                                             aTGGGACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTgaga                    <  1:3741453/71‑1 (MQ=255)

                                                ggACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACa                  <  1:1903834/70‑1 (MQ=255)

                                                 gACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTgaga                    >  1:3991718/1‑67 (MQ=255)

                                                 gACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTgaga                    >  1:3959530/1‑67 (MQ=255)

                                                 gACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAg                 >  1:3990879/1‑70 (MQ=255)

                                                 gACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAg                 >  1:2076418/1‑70 (MQ=255)

                                                 gACGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGt                <  1:1955390/71‑1 (MQ=255)

                                                  aCGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTgaga                    >  1:1249639/1‑66 (MQ=255)

                                                   cGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGa                               >  1:3287798/1‑54 (MQ=255)

                                                   cGCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTAt              <  1:238931/71‑1 (MQ=255)

                                                    gCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATc                           <  1:621576/57‑1 (MQ=255)

                                                    gCCTGACTGAATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATc                           <  1:2707334/57‑1 (MQ=255)

                                                     gctgactgtATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAAGGGAAATCtt                         <  1:142979/57‑1 (MQ=255)

                                                      ctgactgaATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATccc           <  1:2210620/71‑1 (MQ=255)

                                                      ctgactgaATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATccc           <  1:2215728/71‑1 (MQ=255)

                                                       tgactgaTTCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCt          >  1:2956380/1‑71 (MQ=255)

                                                        gagtgaATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCt          >  1:1319671/4‑70 (MQ=255)

                                                         actgaATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCt          <  1:3725734/69‑1 (MQ=255)

                                                         actgaATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCt          <  1:3621272/69‑1 (MQ=255)

                                                         actgaATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGa        <  1:160911/71‑1 (MQ=255)

                                                         actgaATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGa        <  1:3117070/71‑1 (MQ=255)

                                                          ctgaATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTAt              <  1:1679992/64‑1 (MQ=255)

                                                          ctgaATCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGa        >  1:306947/1‑70 (MQ=255)

                                                             aaTCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCAt                                        <  1:2929457/35‑1 (MQ=255)

                                                             aaTCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCttt                        <  1:2678588/51‑1 (MQ=255)

                                                             aaTCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGACAGc    >  1:3783876/1‑71 (MQ=255)

                                                             aaTCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGACAGc    >  1:748352/1‑71 (MQ=255)

                                                             aaTCAGTCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGACAGc    <  1:2121338/71‑1 (MQ=255)

                                                               tcagtcagCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGACAGcc   >  1:942843/1‑70 (MQ=255)

                                                               tcagtcagCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGACAGCCt  <  1:3025573/71‑1 (MQ=255)

CCATTTCTATGGACTCCCATCCGCCCATTTCCTGAAGCACTGATAATGGGACGCCTGACTGAATCAGCCAGCTTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTTGAGACAGTATCCCTGACAGCCT  >  minE/364018‑364151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`