Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	442,228	C→A	A512S (GCG→TCG)	speF ←	ornithine decarboxylase isozyme, inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	442,228	0	C	A	100.0%	289.8 / NA	91	A512S (GCG→TCG)	speF	ornithine decarboxylase isozyme, inducible
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (37/54);  total (37/54)

GCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAG  >  minE/442160‑442295                                                                     |                                                                    gCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGAtt                                                                   <  1:221648/71‑1 (MQ=255) gCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGAtt                                                                   <  1:2603599/71‑1 (MQ=255) gCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGAtt                                                                   <  1:71248/71‑1 (MQ=255)  caattttCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGAtt                                                                   >  1:1245477/4‑70 (MQ=255)  cATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCATGAATCGTCGCGTGTACACCGAACGAtt                                                                   <  1:306549/70‑1 (MQ=255)   aTTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCa                                                                 >  1:692165/1‑71 (MQ=255)   aTTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCa                                                                 >  1:2495821/1‑71 (MQ=255)      tttCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCa                                                                 >  1:2423935/1‑68 (MQ=255)       ttCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCatat                                                              <  1:2367073/70‑1 (MQ=255)       ttCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATAtt                                                             >  1:1527185/1‑71 (MQ=255)          cGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTGCACCGAACGATTCATATTCg                                                           <  1:584745/70‑1 (MQ=255)          cGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCg                                                           <  1:20338/70‑1 (MQ=255)          cGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCg                                                           <  1:2956651/70‑1 (MQ=255)             cACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGt                                                       >  1:1807618/1‑71 (MQ=255)             cACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGt                                                       >  1:3801519/1‑71 (MQ=255)                tACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTAc                                                    >  1:3554436/1‑71 (MQ=255)                 aCGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACg                                                   >  1:663022/1‑71 (MQ=255)                  cGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTAc                                                    <  1:1819898/69‑1 (MQ=255)                  cGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTAc                                                    <  1:3267676/69‑1 (MQ=255)                  cGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGt                                                  >  1:3293439/1‑71 (MQ=255)                  cGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGt                                                  >  1:3575753/1‑71 (MQ=255)                   gCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTg                                                 >  1:1292689/1‑71 (MQ=255)                     cATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCa                                               >  1:3829710/1‑71 (MQ=255)                     cATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCa                                               <  1:3859170/71‑1 (MQ=255)                     cATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCa                                               <  1:549519/71‑1 (MQ=255)                       ttttCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATAtt                                                             <  1:1096841/55‑1 (MQ=255)                       ttttCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATAtt                                                             >  1:1147265/1‑55 (MQ=255)                       ttttCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCAt                                              >  1:454605/1‑70 (MQ=255)                       ttttCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCAt                                              >  1:873643/1‑70 (MQ=255)                       ttttCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCAt                                              >  1:2068280/1‑70 (MQ=255)                         ttCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCg                                                        >  1:3538515/1‑58 (MQ=255)                         ttCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCg                                                        >  1:259279/1‑58 (MQ=255)                         ttCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCg                                                        >  1:1348563/1‑58 (MQ=255)                           cgcgCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATAtt                                                             <  1:2617204/51‑1 (MQ=255)                           cgcgCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATAtt                                                             <  1:3927463/51‑1 (MQ=255)                           cgcgCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCaa                                           <  1:237911/69‑1 (MQ=255)                           cgcgCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAAt                                          <  1:1225573/70‑1 (MQ=255)                           cgcgCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAAt                                          <  1:436300/70‑1 (MQ=255)                              gcAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCt                                      <  1:2303074/71‑1 (MQ=255)                              gcAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCt                                      <  1:1223988/71‑1 (MQ=255)                              gcAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCt                                      <  1:4032577/71‑1 (MQ=255)                               cAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATAcc                                       >  1:1179205/1‑69 (MQ=255)                               cAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTg                                     >  1:96079/1‑71 (MQ=255)                                aGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGc                                                <  1:726319/59‑1 (MQ=255)                                aGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGc                                                <  1:2009730/59‑1 (MQ=255)                                aGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTgg                                    <  1:20432/71‑1 (MQ=255)                                 ggAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCaa                                           >  1:1882040/1‑63 (MQ=255)                                  gAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGAtt                                                                   <  1:1435189/38‑1 (MQ=255)                                     gTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTg                               <  1:3017506/71‑1 (MQ=255)                                     gTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTg                               <  1:1211919/71‑1 (MQ=255)                                     gTTAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTg                               <  1:3110001/71‑1 (MQ=255)                                       tAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGt                              >  1:1338021/1‑70 (MQ=255)                                       tAGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGt                              >  1:333413/1‑70 (MQ=255)                                        aGCAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTg                                     <  1:3788025/62‑1 (MQ=255)                                        aGCAAGAATAGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGt                              >  1:2112233/1‑69 (MQ=255)                                          cAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCa                                            <  1:1114965/53‑1 (MQ=255)                                          cAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGc                          <  1:3999291/71‑1 (MQ=255)                                          cAAGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGAGCATCAATAcc                                       <  1:3054304/58‑1 (MQ=255)                                           aaGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGc                          >  1:1972452/1‑70 (MQ=255)                                            aGAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGc                          <  1:1330466/69‑1 (MQ=255)                                             gAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAAt                                          <  1:2843386/52‑1 (MQ=255)                                             gAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGt                                 >  1:3057628/1‑61 (MQ=255)                                             gAATCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCaa                        >  1:2292770/1‑70 (MQ=255)                                              aaTCGTCGCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCaa                        >  1:2401794/1‑69 (MQ=255)                                                tcgtcgCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGt                    <  1:1111202/71‑1 (MQ=255)                                                tcgtcgCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGt                    <  1:3329554/71‑1 (MQ=255)                                                tcgtcgCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGt                    <  1:2282399/71‑1 (MQ=255)                                                  gtcgCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGttt                  <  1:3157590/71‑1 (MQ=255)                                                  gtcgCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGtt                   <  1:3362035/70‑1 (MQ=255)                                                   tcgCGGGTACACCGAACGATTCATATTCGCCGTTACGTTCATCAATACCTGGGGt                                 <  1:493909/55‑1 (MQ=255)                                                   tcgCGGGTACACCGAACGATTCATATTCGCCGTTACGTGCATc                                             <  1:3234774/43‑1 (MQ=255)                                                     gcgGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTg                 >  1:222383/1‑69 (MQ=255)                                                     gcgGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGc                <  1:814490/70‑1 (MQ=255)                                                      cgGGTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCAt              <  1:4138112/71‑1 (MQ=255)                                                       gggTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATg             >  1:2286519/1‑71 (MQ=255)                                                        ggTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATgg            <  1:2272211/71‑1 (MQ=255)                                                        ggTACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATgg            <  1:1660180/71‑1 (MQ=255)                                                          tACACCGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGAt          <  1:1831801/71‑1 (MQ=255)                                                            cacCGAACGATTCATATTCGCCGTTACGTGCATCaa                                           <  1:988970/36‑1 (MQ=255)                                                              ccGAACGATTCATATTCGCCGTTACGTGCATCAATACCTTGGGTTGt                              <  1:978124/47‑1 (MQ=255)                                                              ccGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGt                              <  1:3606682/47‑1 (MQ=255)                                                              ccGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCa               >  1:2711851/1‑62 (MQ=255)                                                               cGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGa       >  1:476820/1‑69 (MQ=255)                                                               cGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGAc      >  1:2876117/1‑70 (MQ=255)                                                               cGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGAc      >  1:2691731/1‑70 (MQ=255)                                                               cGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACa     <  1:3715918/71‑1 (MQ=255)                                                               cGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACa     <  1:2602554/71‑1 (MQ=255)                                                               cGAACGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACa     <  1:2498735/71‑1 (MQ=255)                                                                 aaCGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTg                 <  1:2241926/57‑1 (MQ=255)                                                                  aCGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCAt              >  1:1128860/1‑59 (MQ=255)                                                                  aCGATTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAg  <  1:3015596/71‑1 (MQ=255)                                                                     |                                                                    GCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAG  >  minE/442160‑442295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑