| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,209,770 | Δ2 bp | coding (167‑168/567 nt) | yebN → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,209,770 | 0 | G | . | 100.0% | 526.8 / NA | 110 | coding (167/567 nt) | yebN | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (110/0); total (110/0) | |||||||||||
| * | minE | 1,209,771 | 0 | A | . | 75.9% | 342.8 / 154.2 | 145 | coding (168/567 nt) | yebN | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/35); new base . (110/0); total (110/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.04e-34 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 | |||||||||||
Only 100 of 145 total aligned reads displayed.
GCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGAT > minE/1209714‑1209836 || gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGCCGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:96565/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:469411/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:178435/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:427189/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:420897/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:397296/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:349912/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:343902/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:334687/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:303319/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:287997/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:282395/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:269618/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:261799/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:240336/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1001099/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:232563/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:216400/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:211159/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:207690/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:200166/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:235796/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:680181/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:914328/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:910787/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:901922/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:897758/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:806577/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:794975/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:764135/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:751515/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:693191/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:688366/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:476197/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:676524/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:653521/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:649121/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:646504/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:634496/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:605553/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:562083/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:501423/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:496449/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1159914/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1345806/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:130025/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1287029/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:127162/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1254816/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1251006/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:122069/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1185080/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1175303/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:171294/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1149688/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1140614/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1108710/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:10972/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1071227/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1014845/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1002064/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1001845/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1347726/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:17408/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1545426/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1502237/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1486289/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1485616/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1466748/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1465346/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1463011/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1383056/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1413533/1‑70 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTag > 1:564230/1‑69 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTag > 1:142379/1‑69 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTag > 1:1019884/1‑69 (MQ=255)gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTCACGCCGCTGATCGGCTGGG‑‑ATGGGCATGTTagc > 1:1039876/1‑70 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCTCTGGATTGCGTTTGTGCTGCTGat < 1:433971/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:777975/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:1522740/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:1542131/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:802132/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:878758/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:886907/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:1016388/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:1555718/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:949999/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:420836/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:217223/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:758603/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:572420/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:1270511/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:1160917/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:658185/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:122154/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:634047/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:1440011/69‑1 (MQ=255) gggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:587755/69‑1 (MQ=255) ggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGTATTGCGTTTGTGCTGCTGat < 1:643921/68‑1 (MQ=255) ggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat < 1:1264577/68‑1 (MQ=255) || GCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGAT > minE/1209714‑1209836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |