Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,301,728	Δ1 bp	coding (68/474 nt)	sbmC ←	DNA gyrase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,301,727	0	T	.	100.0%	32.0 / NA	8	coding (69/474 nt)	sbmC	DNA gyrase inhibitor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (0/8);  total (0/8)

ACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGG  >  minE/1301690‑1301761                                      |                                   acaTCATCGACTGCTCAAAGCCTTTCTTTACCGTCTG‑TCCCACGGGCCAACGAGATGGAAACCTGCAACgg  <  1:763464/71‑1 (MQ=255) acaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTG‑TCCCACGGGCCAACGAGATGGAAACCTGCAACgg  <  1:1059810/71‑1 (MQ=255) acaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTG‑TCCCACGGGCCAACGAGATGGAAACCTGCAACgg  <  1:153942/71‑1 (MQ=255) acaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTG‑TCCCACGGGCCAACGAGATGGAAACCTGCAACgg  <  1:40974/71‑1 (MQ=255) acaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTG‑TCCCACGGGCCAACGAGATGGAAACCTGCAACgg  <  1:596304/71‑1 (MQ=255) acaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTG‑TCCCACGGGCCAACGAGATGGAAACCTGCAACgg  <  1:767062/71‑1 (MQ=255) acaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTG‑TCCCACGGGCCAACGAGATGGAAACCTGCAACgg  <  1:815610/71‑1 (MQ=255) acaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTG‑TCCCACGGGCCAACGAGATGGAAACCTGCAACgg  <  1:914859/71‑1 (MQ=255)                                      |                                   ACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGG  >  minE/1301690‑1301761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑