Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	800,671	+T	coding (658/1419 nt)	dhaH ←	fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	800,668	1	.	T	100.0%	50.3 / NA	15	G221R (GGG→AGG)	dhaH	fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (15/0);  total (15/0)

AAGCGATCAGTGCCTCTTCAGCTTCTGGCCC‑TTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTG  >  minE/800638‑800707                                |                                        aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAg          >  1:104642/1‑63 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACtt   >  1:130701/1‑70 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:1276409/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:1361133/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:1514722/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:271563/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:286299/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:468472/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:529756/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:693869/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:70023/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:714156/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:781315/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:798529/1‑71 (MQ=255) aaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg  >  1:950563/1‑71 (MQ=255)                                |                                        AAGCGATCAGTGCCTCTTCAGCTTCTGGCCC‑TTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTG  >  minE/800638‑800707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑