Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,147,347	+T	coding (150/351 nt)	chbA ←	N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,147,343	1	.	T	100.0%	50.1 / NA	15	A52T (GCC→ACC)	chbA	N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (15/0);  total (15/0)

GCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGC‑TTTTGCTGCGGCAAAATCGCCCTGCTTCGC  >  minE/1147304‑1147373                                         |                               gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCg   >  1:190355/1‑70 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:103122/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:1084375/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:127361/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:1293260/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:1450732/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:1496252/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:208757/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:210472/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:317305/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:697753/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:725635/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:747921/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:823042/1‑71 (MQ=255) gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTTGCTGCGGCAAAATCGCCCTGCTTCGc  >  1:930703/1‑71 (MQ=255)                                         |                               GCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGC‑TTTTGCTGCGGCAAAATCGCCCTGCTTCGC  >  minE/1147304‑1147373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑