Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,602,049	+C	coding (1700/2043 nt)	prlC →	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,602,046	1	.	C	96.7%	100.0 / ‑5.5	30	R566P (CGC→CCC)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base C (29/0);  minor base A (1/0);  total (30/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCG‑CCCGGATCAGGGG  >  minE/2601990‑2602059                                                          |              tATTCTGCTTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1260699/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCg                >  1:1173952/1‑57 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:809395/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:299747/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:363005/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:507331/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:517329/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:560998/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:586728/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:642616/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:732245/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1561429/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:83172/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:890885/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:916450/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:968382/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:968588/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1015180/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:147470/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1416297/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1382641/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1377465/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:137679/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1375774/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1354984/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1187169/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:117647/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:1175822/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:110944/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGCCCCGGATCAgggg  >  1:110213/1‑71 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCGACCCGGATCAggg   >  1:18430/1‑70 (MQ=255) tATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCG‑ccccgc         >  1:1477015/1‑60 (MQ=255)                                                          |              TATTCTGCGTCAGCTGGAGTTCGGCCTGTTTGATTTCCGCCTTCATGCCGAGTTCCG‑CCCGGATCAGGGG  >  minE/2601990‑2602059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑