Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,677,6950CT57.9% 101.8 / 206.6 171intergenic (‑145/+4)ndk/pbpCmultifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase/fused transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/72);  new base T (0/99);  total (0/171)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 171 total aligned reads displayed.

TGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAA  >  minE/1677640‑1677755
                                                       |                                                            
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:294599/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:403721/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:790311/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:369940/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:791541/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:795391/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:341795/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:329998/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:323359/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:77013/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:290226/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:270190/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:805138/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:229239/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:816156/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:825275/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1545034/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1525987/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:565828/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:657168/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:652104/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:645125/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:677639/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:624695/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:602122/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:71272/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:583024/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1528788/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:7205/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:541093/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:525408/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:477850/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:476955/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:457789/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:74976/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1499588/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1049272/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:916652/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1117300/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1130084/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1161421/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:117016/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1173909/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:118373/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:884998/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1239181/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:125857/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1265694/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1271964/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:874059/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1334099/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:836147/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:844259/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1484537/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1472823/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1449553/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:858198/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1412207/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1400235/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1359965/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1349305/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:868080/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1341859/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1309509/63‑1 (MQ=255)
tgaAGGTAACATTTTTTTAAAATAAACCCAGTTCAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:1122304/63‑1 (MQ=255)
tgaAGGTAACAATTTTTTAAAATAAACCCAGTTTAAGCAACAATCAGGGACAAAATAGACTAt                                                       <  1:744458/63‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:89385/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:748575/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:346649/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:692768/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:922880/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:934683/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:952154/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:962450/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:180040/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:101739/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1024662/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1059638/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1086570/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:120127/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1216591/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1301274/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1344131/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1434073/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1505750/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:636548/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:196046/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:230664/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:344110/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1008996/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:424986/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:446542/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:449527/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:489697/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:553300/71‑1 (MQ=255)
                                             aGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:616255/71‑1 (MQ=255)
                                              gggACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1496717/70‑1 (MQ=255)
                                              gggACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:370200/70‑1 (MQ=255)
                                              gggACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:1041365/70‑1 (MQ=255)
                                              gggACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa  <  1:584460/70‑1 (MQ=255)
                                                       |                                                            
TGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAA  >  minE/1677640‑1677755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: