| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,124,172 | 0 | C | A | 65.2% | 160.1 / 181.0 | 158 | Q38K (CAA→AAA) | yhbO | predicted intracellular protease |
| Reads supporting (aligned to +/- strand): ref base C (0/55); new base A (0/103); total (0/158) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 158 total aligned reads displayed.
AGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAA > minE/2124108‑2124240 | aGAATTTACTTCACCCGCAGACGAGTTGCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1048896/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGTTTACCATTGAAAAAAAAGCg < 1:859598/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:257936/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:329272/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:320957/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:760634/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:306881/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:774057/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:292940/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:284150/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:743105/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:240184/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:236428/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:217259/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:778415/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:200064/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:194049/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:797540/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:730588/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:645311/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:639509/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:639211/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:651346/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:534702/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:527392/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:492435/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:481604/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:334468/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:464298/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:426077/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:402138/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:391143/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:374294/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:366235/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1554064/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1103771/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:889662/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1245021/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:799557/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1221000/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1193127/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1167300/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1139980/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1124307/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:89204/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1099866/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:932018/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1086873/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:94255/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:943509/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1003112/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1018491/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:879213/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:825616/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1494322/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1454843/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1440856/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1434421/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1406090/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1403577/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1390313/70‑1 (MQ=255)aGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:1371901/70‑1 (MQ=255) aTTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:849051/67‑1 (MQ=255) agCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:448223/54‑1 (MQ=255) aCGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:909932/50‑1 (MQ=255) aCGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAAAAAGCg < 1:277422/50‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:897473/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:713967/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:854285/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:712681/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:678960/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:882789/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:983959/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:806527/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1452939/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:105564/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1081079/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1083029/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1098430/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1161477/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1213982/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:122222/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1292892/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1303627/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1315663/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1356530/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1380444/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:619417/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1525121/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1526953/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:153352/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:1543790/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:204742/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:295492/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:320872/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:359037/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:47361/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:532924/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:56159/71‑1 (MQ=255) aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa < 1:597620/71‑1 (MQ=255) | AGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAA > minE/2124108‑2124240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |