| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,148,099 | 0 | G | A | 38.8% | 98.0 / 141.0 | 129 | N355N (AAC→AAT) | glmM | phosphoglucosamine mutase |
| Reads supporting (aligned to +/- strand): ref base G (79/0); new base A (50/0); total (129/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 129 total aligned reads displayed.
TCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCG > minE/2148050‑2148150 | tttGCGTGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1979/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACgcgc > 1:1142565/3‑58 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCac > 1:263205/3‑69 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1314501/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:623465/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1338972/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:650970/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:589325/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1398320/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1404277/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1410489/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1426791/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1436218/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:538585/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1481939/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1485267/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:537861/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:532170/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:500694/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:185685/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:444410/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:191666/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:250501/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:306077/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1137900/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:974367/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:966662/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:959149/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1036774/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:842593/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:834750/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1082138/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1108153/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1271091/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1144729/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:752003/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1182037/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1190716/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:721136/3‑70 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAGc > 1:218374/3‑71 (MQ=255)tttGCGGGAACATTTTCATGCCGCTGCAAAGCTCGTGCAGGCTCATATGATTACGCGCCATCGCCGCCAg > 1:1101511/3‑70 (MQ=255) ggTCGTGCAGTCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:439481/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTTCGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCg > 1:145252/1‑65 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCAcc > 1:33480/1‑44 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGt > 1:1338369/1‑66 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCAcc > 1:1251581/1‑70 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCAcc > 1:1259205/1‑70 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:697831/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:338529/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:668424/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:713148/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:379540/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:320408/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:407323/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:411596/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:892867/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:424557/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:657038/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:464480/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:186053/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:715282/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:572446/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:809626/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:544826/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:734549/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1210173/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1369957/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1344878/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1338268/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1331130/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1326532/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1263966/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1224707/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1217890/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1374356/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1173745/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1166340/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1071213/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1063920/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1023066/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1012114/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1010374/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1005927/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1385308/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:287626/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:261835/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:254943/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:24037/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:214703/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:200062/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:192712/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:190414/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:164932/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1518717/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1497271/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1486945/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1440177/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1431004/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:295949/1‑71 (MQ=255) ggTCGTGCAGGCTCATATGGTTACGCGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCg > 1:1001546/1‑71 (MQ=255) | TCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGGTTACGTGCCATCGCCGCCAGCACCTGCAAGCCAGCAACGATGCCGTCACCG > minE/2148050‑2148150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |