| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,222,053 | 0 | T | C | 72.2% | 246.3 / 118.1 | 158 | Y285Y (TAT→TAC) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/44); new base C (0/114); total (0/158) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 158 total aligned reads displayed.
GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGG > minE/2222042‑2222112 | ggCGAGTTCTACTTCATCGAGATTAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:396811/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:347881/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:543282/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:50957/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:494905/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:487602/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:470605/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:454362/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:423250/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:401977/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:347947/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:547474/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:346872/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:312398/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:288189/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:274107/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:24786/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:243073/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:222716/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:214536/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:190317/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:824231/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:983396/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:965188/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:916549/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:892039/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:874460/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:865344/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:858030/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:833518/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:831903/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1014416/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:77871/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:763706/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:707629/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:704046/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:666026/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:639039/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:599268/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:589694/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:56155/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1171937/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1198848/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1220521/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1238173/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1187673/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1292332/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:189484/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1319571/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1343272/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1343849/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1379109/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1384099/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1404411/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:117906/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1443531/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1174849/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1139052/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:185820/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1134164/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:180782/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:105835/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1038224/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1036752/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1487288/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1548495/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:163416/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1007974/71‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:993404/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:692742/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:98387/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:1030880/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:867463/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:1123581/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:758775/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:55730/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:1153770/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:146614/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:207236/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:237912/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:1552562/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:24804/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:1486428/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:324049/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:337294/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:653747/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:1464585/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:1454448/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:46606/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:562409/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:123469/39‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1449333/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:861187/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1481105/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1444673/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:426350/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:939062/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:689428/70‑1 (MQ=255) cGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1023208/69‑1 (MQ=255) gAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:407404/37‑1 (MQ=255) | GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGG > minE/2222042‑2222112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |