Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,222,0620AG72.3% 235.9 / 128.1 159E288E (GAA→GAG) accCacetyl‑CoA carboxylase, biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/44);  new base G (0/115);  total (0/159)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 159 total aligned reads displayed.

GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGG  >  minE/2222042‑2222112
                    |                                                  
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:315549/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:50957/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:49069/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:487602/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:470087/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:454362/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:425336/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:423250/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:401977/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:347947/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:347881/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:543282/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:312398/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:278987/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:248197/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:246815/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:243073/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:22343/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:222254/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:214536/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:199745/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:824231/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:983396/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:965188/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:916549/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:892039/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:874460/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:865344/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:854572/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:833518/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:831903/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1014416/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:77871/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:707629/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:704046/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:666026/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:646911/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:639039/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:599268/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:589694/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:546693/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:186553/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1220521/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1238173/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1198848/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1187673/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:117906/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1292332/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:132216/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1343272/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1356259/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1384099/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1176300/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1389298/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1443531/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1171937/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1139052/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:185820/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1134164/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:190317/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1007974/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:105835/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1036752/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1469985/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1038224/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1487288/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGGCACCGAGATGATCACCgg  <  1:3672/71‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:867463/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:1030880/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:758775/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:692742/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:98387/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:1123581/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:1153770/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:993404/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:562409/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:169346/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:159215/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:1552562/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:1537636/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:24804/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:146614/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:337294/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:345391/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:1454448/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:403068/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:1441694/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:46606/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:125865/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:55730/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:560675/39‑1 (MQ=255)
 gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:1235196/39‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1463806/70‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:861187/70‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1482709/70‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:939062/70‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:689428/70‑1 (MQ=255)
  cGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:1446616/38‑1 (MQ=255)
  cGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg                                 <  1:762683/38‑1 (MQ=255)
  cGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg  <  1:1023208/69‑1 (MQ=255)
                    |                                                  
GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGG  >  minE/2222042‑2222112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑