| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,222,075 | 0 | A | G | 44.3% | 118.8 / inf | 262 | I293V (ATT→GTT) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit |
| Reads supporting (aligned to +/- strand): ref base A (102/44); new base G (0/116); total (102/160) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.16e-38 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 262 total aligned reads displayed.
GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGC > minE/2222042‑2222145 | ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:470087/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:694005/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1404411/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:666026/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1443531/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:63878/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1469985/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:599268/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1487288/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:547474/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:49069/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1548495/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1379109/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:423250/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:401977/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:185820/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:199745/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:222254/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:22343/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:24786/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:248197/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:278987/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:315549/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1174849/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1014416/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:974339/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1036752/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:105835/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:106011/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:892039/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:874460/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1134164/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:854572/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1343272/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1180330/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1187673/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:831903/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1198848/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1220521/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:77871/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:763706/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:750863/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:707629/71‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:560675/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:863948/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:34794/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:653747/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:758775/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:345391/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:993404/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:98387/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:169346/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:159215/39‑1 (MQ=255) gCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:1235196/39‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1481105/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1463806/70‑1 (MQ=255) cGAGTTCTATTTCATCGAAATGAACACCCGTATTCAgg < 1:1446616/38‑1 (MQ=255) cGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1023208/69‑1 (MQ=255) gAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:571522/68‑1 (MQ=255) aTGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:189579/50‑1 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGCTCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:446367/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCGCCGGCGtt > 1:1302492/1‑44 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGCCCTGATCAAAGAACAGCTGCGTAt > 1:1108094/1‑70 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGATCAGCTGCGTATc > 1:815253/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTg > 1:394232/1‑65 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt > 1:475052/1‑70 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt > 1:363070/1‑70 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt > 1:1252961/1‑70 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1086686/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:80517/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1243962/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1191385/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:832619/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1156153/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1006654/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:90732/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:927003/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:931163/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:948050/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1033808/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:303938/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1350991/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:293332/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:333059/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:23885/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:167335/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:442706/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:461763/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1544911/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:502139/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:539137/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:545560/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1532250/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1507102/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:586484/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1417739/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:687616/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1267105/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGATACAGAAATGATCAc > 1:1153151/1‑37 (MQ=255) gTATTCAGGT‑‑AACACCCGGTTACAGAAAAGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGc > 1:866406/1‑71 (MQ=255) | GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGC > minE/2222042‑2222145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |