| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,222,083 | 0 | A | C | 53.7% | 100.7 / 307.7 | 219 | V295V (GTA→GTC) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit |
| Reads supporting (aligned to +/- strand): ref base A (101/0); new base C (0/117); total (102/117) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 219 total aligned reads displayed.
GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGC > minE/2222042‑2222145 | ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:246815/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:49069/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:470087/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:454362/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:423250/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:347947/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:346872/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:288189/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:278987/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:509591/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:22343/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:222254/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:199745/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:189484/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:185820/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1548495/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1487288/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1014416/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:546693/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:63878/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:646911/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:680335/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:707629/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:763706/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:824231/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:832273/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:833518/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:854572/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:865344/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:874460/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:906459/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:916549/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:974339/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1134164/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1319571/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1343272/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1292332/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:105835/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1038224/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1389298/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1238173/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1220521/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1198848/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1171937/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1187673/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1180330/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1176300/71‑1 (MQ=255)ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGGCACCGAGATGATCACCgg < 1:3672/71‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:689428/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:426350/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1481105/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:939062/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1463806/70‑1 (MQ=255) gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1444673/70‑1 (MQ=255) cGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:1023208/69‑1 (MQ=255) gAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg < 1:571522/68‑1 (MQ=255) gTATTCAGGTAGAACTCCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:660178/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTATAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1201131/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGCCCTGATCAAAGAACAGCTGCGTAt > 1:1108094/1‑70 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGCCCTGATCAAAGAACAGCTGCGTAt > 1:30194/1‑70 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAg > 1:478976/1‑62 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTg > 1:394232/1‑65 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt > 1:1244412/1‑70 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt > 1:1252961/1‑70 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt > 1:172636/1‑70 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1006654/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:990736/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:960988/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:737920/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:502139/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:756256/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:775215/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:787740/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:931163/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:809742/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1034247/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:890614/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1074386/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1086686/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:272120/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1493954/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1508957/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:15441/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:160177/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:167335/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1438284/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1405894/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1361995/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:23885/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1350991/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:594213/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:309946/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:333059/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:445319/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:461763/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:540309/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:1190351/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:549621/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc > 1:586484/1‑71 (MQ=255) gTATTCAGGTAGAACACCCGGATACAGAAATGATCAc > 1:1153151/1‑37 (MQ=255) | GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGC > minE/2222042‑2222145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |