Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,222,0950TC53.2% 6.6 / inf 219V299V (GTT→GTC) accCacetyl‑CoA carboxylase, biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base T (102/0);  new base C (0/116);  total (102/117)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 219 total aligned reads displayed.

GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGC  >  minE/2222042‑2222145
                                                     |                                                  
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:246815/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:49069/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:470087/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:454362/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:423250/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:347947/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:346872/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:288189/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:278987/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:509591/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:22343/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:222254/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:199745/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:189484/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:185820/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1548495/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1487288/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1014416/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:546693/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:63878/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:646911/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:680335/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:707629/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:763706/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:824231/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:832273/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:833518/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:854572/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:865344/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:874460/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:906459/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:916549/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:974339/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1134164/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1319571/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1343272/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1292332/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:105835/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1038224/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1389298/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1238173/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1220521/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1198848/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1171937/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1187673/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1180330/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1176300/71‑1 (MQ=255)
ggCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGGCACCGAGATGATCACCgg                                   <  1:3672/71‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:689428/70‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:426350/70‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1481105/70‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:939062/70‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1463806/70‑1 (MQ=255)
 gCGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1444673/70‑1 (MQ=255)
  cGAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:1023208/69‑1 (MQ=255)
   gAGTTCTACTTCATCGAGATGAACACCCGCGTCCAGGTCGAACACCCGGTCACCGAGATGATCACCgg                                   <  1:571522/68‑1 (MQ=255)
                               gTATTCAGGTAGAACTCCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:660178/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTATAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1201131/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGCCCTGATCAAAGAACAGCTGCGTAt     >  1:1108094/1‑70 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGCCCTGATCAAAGAACAGCTGCGTAt     >  1:30194/1‑70 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAg             >  1:478976/1‑62 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTg          >  1:394232/1‑65 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt     >  1:1244412/1‑70 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt     >  1:1252961/1‑70 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTAt     >  1:172636/1‑70 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1006654/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:990736/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:960988/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:737920/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:502139/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:756256/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:775215/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:787740/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:931163/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:809742/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1034247/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:890614/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1074386/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1086686/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:272120/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1493954/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1508957/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:15441/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:160177/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:167335/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1438284/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1405894/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1361995/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:23885/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1350991/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:594213/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:309946/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:333059/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:445319/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:461763/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:540309/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:1190351/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:549621/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATc    >  1:586484/1‑71 (MQ=255)
                               gTATTCAGGTAGAACACCCGGATACAGAAATGATCAc                                      >  1:1153151/1‑37 (MQ=255)
                                                     |                                                  
GGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGC  >  minE/2222042‑2222145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑