Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,339,8710TC35.3% 280.7 / 252.6 255V112V (GTA→GTG) bipAGTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/165);  new base C (0/90);  total (0/255)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 264 total aligned reads displayed.

TGGTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGA  >  minE/2339867‑2339939
    |                                                                    
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:404741/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1381372/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1424534/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:144048/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1515641/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1547430/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:182306/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:207220/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:236517/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:272243/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:293288/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:347268/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:377706/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1022158/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:426908/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:467168/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:491669/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:52999/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:61911/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:669351/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:700671/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:730719/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:900662/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:939370/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:992497/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1235290/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1117381/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1245657/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1132964/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1087272/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1046408/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1274400/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1295832/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1153790/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1483220/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:855849/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:162888/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:156733/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:449908/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1413576/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:325167/70‑2 (MQ=255)
  gTTACGTAGCGCGTTTGCGGCATGGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:357123/71‑1 (MQ=255)
  gTTACGTAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:675633/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGTGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:769696/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:722361/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:708365/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:826598/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1077764/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:69763/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:642489/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1101280/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:615653/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:582173/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1074275/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:737843/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:766238/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:790131/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1147024/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:840641/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:868280/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:884321/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:893518/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:906985/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:927742/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1041139/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:954076/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:968636/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:980085/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1008775/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1232312/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1370078/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1378423/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1347901/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1397015/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:132309/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1315144/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1450229/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1461697/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1473011/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:148970/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1502141/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1256857/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:213188/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:252638/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:263446/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:579321/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:287129/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:28793/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1195729/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:294431/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1182186/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1158677/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:397133/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1152905/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:492269/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:509141/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:574596/71‑1 (MQ=255)
   ttACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1359111/70‑1 (MQ=255)
   ttACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:526622/70‑1 (MQ=255)
   ttACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:607973/70‑1 (MQ=255)
    |                                                                    
TGGTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGA  >  minE/2339867‑2339939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑