Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,339,8800CG34.8% 352.7 / 303.1 268T109T (ACG→ACC) bipAGTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/174);  new base G (0/93);  total (0/268)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 269 total aligned reads displayed.

TGGTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGA  >  minE/2339867‑2339939
             |                                                           
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:426908/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:140201/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1424534/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:144048/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1515641/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1547430/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:182306/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:207220/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:236517/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:272243/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:293288/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:347268/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:404741/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1022158/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:467168/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:491669/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:579136/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:61911/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:700671/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:730719/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:764147/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:893123/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:900662/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:939370/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:992497/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1139076/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1274400/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1295832/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1154413/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1117381/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1245657/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1235290/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1184173/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:813404/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:162888/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:156733/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1064209/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:449908/70‑2 (MQ=255)
  gTTACGTAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:675633/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1101280/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:708365/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:722361/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:785725/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1091267/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:69763/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:658184/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:642489/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:605147/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:582173/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:57320/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:737843/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1077764/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:769617/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1152905/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:840641/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:854374/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:868280/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:884321/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1074275/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:906985/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:921860/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1041139/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:954076/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:965382/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:980085/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1008775/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:29122/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1315144/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1445663/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1461697/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1473011/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1482006/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:148970/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1502141/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1256857/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:213920/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:252638/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:132309/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1232312/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:287129/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1347901/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:314589/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:332057/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:361751/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:388749/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:397133/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1158677/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1384755/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1378423/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1147024/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:503497/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:509141/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1370078/71‑1 (MQ=255)
   ttACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:263447/70‑1 (MQ=255)
   ttACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:526622/70‑1 (MQ=255)
   ttACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1359111/70‑1 (MQ=255)
    tACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:612900/69‑1 (MQ=255)
     aCGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1402570/68‑1 (MQ=255)
        aaGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:530813/63‑2 (MQ=255)
             cgTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1206799/60‑1 (MQ=255)
             |                                                           
TGGTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGA  >  minE/2339867‑2339939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑