Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,339,8830TC34.6% 312.4 / 259.1 269Q108Q (CAA→CAG) bipAGTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/176);  new base C (0/93);  total (0/269)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 270 total aligned reads displayed.

TGGTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGA  >  minE/2339867‑2339939
                |                                                        
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:426908/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1435928/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1487942/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1496205/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1515641/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1547430/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1554501/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:182306/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:236517/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:272243/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:293288/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:347268/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:377706/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:404741/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1022158/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:467168/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:491669/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:52999/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:579136/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:61911/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:700671/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:730719/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:764147/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:834744/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:893123/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:900662/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:939370/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:992497/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1249011/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1381372/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1376109/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1153790/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1132964/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1274400/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1225631/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1064209/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:325167/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:162888/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:813404/70‑2 (MQ=255)
  gTTACGTAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:675633/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:658184/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1101280/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:722361/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:708365/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:683438/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:769617/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:634836/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:605147/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:582173/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1147024/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:57320/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:737843/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1091267/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:492269/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:785725/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1077764/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:854374/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:868280/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:884321/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1074275/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:906985/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:921860/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1041139/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:954076/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:965382/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:980085/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1008775/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:263446/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1397015/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1420594/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1445663/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1455547/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1463758/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1482006/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1370078/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1354381/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1340492/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:132309/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1310899/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:128969/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:19517/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:213188/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:242986/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:287129/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:28793/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1239054/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:314589/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1151778/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1232672/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:361751/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:509141/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1195729/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1182186/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1158677/71‑1 (MQ=255)
  gTTACGAAGCGCGCTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:440479/71‑1 (MQ=255)
   ttACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:526622/70‑1 (MQ=255)
    tACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:612900/69‑1 (MQ=255)
     aCGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:391802/66‑2 (MQ=255)
     aCGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1402570/68‑1 (MQ=255)
               tttGCGGCATCGGGCCGTCAAATGCGTCAAGCACCAGCAGCACTGAGTCTACCATGGa  <  1:1113655/58‑1 (MQ=255)
                |                                                        
TGGTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGA  >  minE/2339867‑2339939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑