Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,339,8980GT33.8% 429.7 / 277.1 275D103E (GAC→GAA) bipAGTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/182);  new base T (0/93);  total (0/275)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-03
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 275 total aligned reads displayed.

TGGTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGA  >  minE/2339867‑2339939
                               |                                         
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:272243/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1435928/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1487942/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1496205/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1515641/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1547430/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1554501/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:182306/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:207220/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:236517/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1022158/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:426908/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:467168/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:491669/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:579136/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:700671/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:730719/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:764147/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:939370/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:992497/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1184173/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1117381/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1132964/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1154413/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1235290/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1249011/70‑2 (MQ=255)
gggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1274400/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:162888/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:855849/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:813404/70‑2 (MQ=255)
 ggTCACGAAGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:1064209/70‑2 (MQ=255)
  gTTACGTAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:675633/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGTGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:769696/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1077764/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:965382/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:683438/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:658184/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:634836/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:616918/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:605147/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:582173/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1091267/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:574596/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:509141/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:503497/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1103844/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:980085/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:708365/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:722361/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:927742/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:107550/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1008775/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:737843/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:954076/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:785725/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:840641/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1041139/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:868280/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:884321/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:893518/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:905094/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:906985/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1384755/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:213188/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1232312/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1482006/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1463758/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1455547/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1445063/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1420594/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1397015/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1158677/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1378423/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1370078/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1354381/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1347901/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:132309/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1310899/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:128969/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:252638/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:397133/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:388749/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1147024/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:287129/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:29122/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:294421/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1152905/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:314589/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:332057/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:356466/71‑1 (MQ=255)
  gTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:373975/71‑1 (MQ=255)
  gTTACGAAGCGCGCTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:440479/71‑1 (MQ=255)
   ttACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:263447/70‑1 (MQ=255)
   ttACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:526622/70‑1 (MQ=255)
    tACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:612900/69‑1 (MQ=255)
     aCGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1402570/68‑1 (MQ=255)
        aaGCGGGTCTGCGGCATCGGGCCTTCCTGCGCATCGACCAGCAGCAGCACCGAGTCGACCAtc    <  1:530813/63‑2 (MQ=255)
             cgTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1206799/60‑1 (MQ=255)
                       aTCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:1243910/50‑1 (MQ=255)
                       aTCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGa  <  1:405297/50‑1 (MQ=255)
                               |                                         
TGGTTACGAAGCGCGTTTGCGGCATCGGGCCGTCAAATGCGTCAACCACCAGCAGCACTGAGTCTACCATGGA  >  minE/2339867‑2339939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑