| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,557,356 | 0 | C | A | 63.9% | 256.3 / inf | 274 | Q4K (CAA→AAA) | secB | protein export chaperone |
| Reads supporting (aligned to +/- strand): ref base C (0/99); new base A (0/175); total (0/274) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 274 total aligned reads displayed.
GGGTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGA > minE/2557334‑2557405 | gggTTTTCTACACATGTCAGAAAAAAACAAGACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1060543/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCATCGTATTTATACCAAgg < 1:1495357/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:309724/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:821152/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1528163/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:793905/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:780234/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:17834/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:758143/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:198538/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:741249/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:2258/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:725643/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:262892/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:264845/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:277881/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:986847/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1016982/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:338640/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:697465/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:658017/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:39899/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:411339/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:422698/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:436568/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:48169/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:496195/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:516229/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:614661/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:577849/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1338723/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1127776/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1133020/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:114680/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1188681/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1210022/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1220579/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1112336/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:959853/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1259582/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1281368/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1098167/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:912208/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:133486/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:838906/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:905697/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:970970/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1388562/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1082784/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1469240/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:14180/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:134331/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:852441/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:847871/71‑1 (MQ=255)gggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1437604/71‑1 (MQ=255)gggTTTTCAACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1546479/71‑1 (MQ=255)gggTTTTCAACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:590988/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCTAGGa < 1:173318/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:648742/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:630915/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:337526/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:650299/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:66295/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:668997/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:719410/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:922253/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:87782/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:867661/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:816577/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:830313/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:543444/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1023807/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1083552/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1237527/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1286743/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1313368/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1362512/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1384604/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1399522/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1425216/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:144875/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1486999/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1518017/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:1533843/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:15417/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:15827/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:186979/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:204658/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:240565/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:368933/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:38545/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:388085/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:391907/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:447259/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:452380/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:473985/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGa < 1:56272/71‑1 (MQ=255) ggTTTTCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:353251/70‑1 (MQ=255) tttCTACACATGTCAGAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:1479687/67‑1 (MQ=255) gAAAAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAgg < 1:802612/52‑1 (MQ=255) | GGGTTTTCTACACATGTCAGAACAAAACAACACTGAAATGACTTTCCAGATCCAACGTATTTATACCAAGGA > minE/2557334‑2557405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |