Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	447,228	C→A	R163L (CGG→CTG)	kdpD ←	fused sensory histidine kinase in two‑component regulatory system with KdpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	447,228	0	C	A	100.0%	291.6 / NA	80	R163L (CGG→CTG)	kdpD	fused sensory histidine kinase in two‑component regulatory system with KdpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (80/0);  total (80/0)

TCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCA  >  minE/447189‑447258                                        |                               tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGt                              >  1:615184/1‑42 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGt                              >  1:736265/1‑42 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGt                >  1:531584/1‑56 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGt                >  1:451684/1‑56 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGAcgccg         >  1:1497922/1‑63 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTg      >  1:994255/1‑66 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGCCca  >  1:1032163/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGCCca  >  1:780997/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGAc    >  1:1224279/1‑68 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:511702/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:358942/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:609647/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:608960/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:320574/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:605095/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:366206/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:514515/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:43774/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:498339/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:498063/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:485408/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:458290/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:377485/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:442248/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:797556/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:95267/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:936235/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:91618/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:890518/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:86811/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:863061/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:85428/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:80513/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:68161/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:782298/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:766762/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:747613/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:72569/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:708449/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:697190/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:695218/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1113908/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1475552/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:146573/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1446460/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1422478/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:129140/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1289365/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1256061/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1227587/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1210783/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1018055/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1097300/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1095612/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1081152/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1061921/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1056024/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1054913/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1031558/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1031133/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:252135/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:342660/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:319186/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:299621/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:275375/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:271199/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:266998/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:265624/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:249533/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:236365/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:222586/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:188237/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1511500/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1482039/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1481921/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGGGACGCCGCTGACca  >  1:419365/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTACGGTGACGCCGCTGACca  >  1:320150/1‑70 (MQ=255) tcgtcgGCGGCATCGAAAAAAGGATCGGGCAAGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGAcaa  >  1:1228346/1‑68 (MQ=255) tcgtcgGCCGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAAt                      >  1:1486399/1‑50 (MQ=255) acgtcgGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCAGTACCTGAATTCCGGTGACGCCGCTGACca  >  1:1479090/2‑70 (MQ=255)                                        |                               TCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAATTCCGGTGACGCCGCTGACCA  >  minE/447189‑447258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑