Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	692,900	T→C	A144A (GCT→GCC)	hyaD →	protein involved in processing of HyaA and HyaB proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	692,900	0	T	C	100.0%	33.7 / NA	11	A144A (GCT→GCC)	hyaD	protein involved in processing of HyaA and HyaB proteins
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/11);  total (0/11)

CTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGG  >  minE/692868‑692938                                 |                                       cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:1187851/71‑1 (MQ=255) cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:1208922/71‑1 (MQ=255) cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:1355180/71‑1 (MQ=255) cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:1359238/71‑1 (MQ=255) cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:1386599/71‑1 (MQ=255) cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:1447388/71‑1 (MQ=255) cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:346032/71‑1 (MQ=255) cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:425311/71‑1 (MQ=255) cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:722979/71‑1 (MQ=255)  tGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:87205/70‑1 (MQ=255)                         cTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgggg  <  1:246243/47‑1 (MQ=255)                                 |                                       CTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGG  >  minE/692868‑692938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑