Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	841,995	A→G	S25S (TCA→TCG)	rssB →	response regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	841,995	0	A	G	100.0%	177.4 / NA	50	S25S (TCA→TCG)	rssB	response regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/50);  total (0/50)

TTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGG  >  minE/841975‑842023                     |                             tttCGCTCGCTTCTGGATTCGTTGTTTTCCTCATTGGGAGCGACAACgg  <  1:378080/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTTGTTTTCCTCATTGGGAGCGACAACgg  <  1:498496/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGTGAGCGACAACgg  <  1:347707/49‑1 (MQ=38) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAActg  <  1:1206434/49‑3 (MQ=39) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:726079/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:380331/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:381951/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:390345/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:401712/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:516404/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:541251/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:563342/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:595375/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:696816/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:9797/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:761787/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:764716/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:77344/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:77649/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:802513/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:80367/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:808310/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:830904/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:865043/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:878403/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1063317/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1548409/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1068045/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1106624/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1139109/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1226279/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1226801/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1232154/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1305836/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1359529/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1452708/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:368159/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1550664/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:210686/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:225056/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:266451/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:270469/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:276598/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:330820/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:356915/49‑1 (MQ=255) tttCGCTCGCTTCTGGATTCGTGGTTTTCCTCATTGGGAGAGACAACgg  <  1:179725/49‑1 (MQ=39) tttCGCTCGCTTCTGGATGCGTGGCTTTCCTCATTGGGAGCGACAACgg  <  1:718773/49‑1 (MQ=255) tttCGCTCGCTGCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:115015/49‑1 (MQ=39)      ctcgctTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:720828/44‑1 (MQ=255)         gctTCTGGATTCGTGGTTTTCCTCATTGGGAGCGACAACgg  <  1:1526604/41‑1 (MQ=255)                     |                             TTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGG  >  minE/841975‑842023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑