| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,158,125 | A→T | F111Y (TTT→TAT) | astA ← | arginine succinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,158,125 | 0 | A | T | 77.8% | 191.8 / 50.6 | 90 | F111Y (TTT→TAT) | astA | arginine succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (20/0); new base T (0/70); total (20/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-20 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
TTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTG > minE/1158058‑1158171 | tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:477108/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:205758/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:645089/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:1229449/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:1266347/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:23283/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:1310078/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:511748/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:787061/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:261125/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:1415008/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:1494063/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:1086365/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:446206/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:444375/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:1541537/1‑69 (MQ=255)tttGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCG‑‑GTGATCGTTACTGAGAAACa > 1:414401/1‑69 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc > 1:630047/1‑48 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCTATGCGTTATAGACATTCAGc > 1:570073/1‑69 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCg > 1:448696/1‑54 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTc > 1:1262807/1‑66 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAg > 1:1041872/1‑68 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:160182/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:1043286/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:895820/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:820316/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:459003/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:1423080/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:1130019/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:1311879/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:1176939/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:553957/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGctc > 1:580915/1‑71 (MQ=255) cacaGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGct > 1:785753/1‑70 (MQ=255) ctgcCGGTGTGATCGTTACTGAGTTACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:412245/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGTGGCGTg < 1:200941/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:329659/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:298278/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:324731/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:352659/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:93026/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:365947/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:427905/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:442299/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:482807/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:492280/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:507927/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:514181/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:619492/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:649351/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:705138/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:762280/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:764568/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:771289/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:794724/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:897352/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:116232/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1327838/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1300144/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:283333/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1266512/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1245192/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1244881/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1222137/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1220557/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1181933/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:127622/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1157445/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1146412/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1142893/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1133251/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:11220/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1096913/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1085008/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1056371/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1479807/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1535125/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1514063/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1513249/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:15458/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:193124/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:210372/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:230888/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:235518/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1460598/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1375569/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:249567/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1363637/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1348842/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1344436/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:276521/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGGCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:744343/71‑1 (MQ=255) ctgcCGGTGTGATCGTTACTGAGATACAGCGCCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1500930/71‑1 (MQ=255) tgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGGCATTCAGCTCTTTTGAGGCGTg < 1:1330932/70‑1 (MQ=255) tgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:659233/70‑1 (MQ=255) tgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:653954/70‑1 (MQ=255) tgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:1247110/70‑1 (MQ=255) tgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:914515/70‑1 (MQ=255) tgcCGGTGTGATCGTTACTGAGATACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:52358/70‑1 (MQ=255) aCTGAGATACAGCGTCGGCATTGCGTTATAGACATTCAGCTCTTTTGAGGCGTg < 1:755330/54‑1 (MQ=255) | TTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCGTG > minE/1158058‑1158171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |