Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,172,937	G→T	A572E (GCG→GAG)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,172,937	0	G	T	100.0%	50.0 / NA	15	A572E (GCG→GAG)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/0);  total (15/0)

CAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAA  >  minE/1172918‑1172975                    |                                       cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCAtttctg          >  1:184996/1‑48 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:1140190/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:1297292/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:130810/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:1350877/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:1538509/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:271434/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:349327/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:433920/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:48153/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:533803/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:599994/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:69706/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa  >  1:963665/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGa   >  1:1514712/1‑57 (MQ=255)                    |                                       CAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAA  >  minE/1172918‑1172975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑