Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,172,966	C→T	P562P (CCG→CCA)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,172,966	0	C	T	100.0%	43.3 / NA	14	P562P (CCG→CCA)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/0);  total (14/0)

CAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAC  >  minE/1172918‑1173036                                                 |                                                                       cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:1350877/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:963665/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:599994/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:271434/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:349327/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:1297292/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:130810/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:1538509/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:69706/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:433920/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:1140190/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:533803/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGaa                                                               >  1:48153/1‑58 (MQ=255) cAGCACCGATTCCCAGTGCTCGGTCATGTCCGGTCGCGTCGCCATTTCTGGCAGCGa                                                                >  1:1514712/1‑57 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:56401/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:509857/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:546102/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:449150/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:568967/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:503198/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:497867/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:483271/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:20951/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:437675/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:431338/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:374/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:364809/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:227405/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:160663/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:738826/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:976205/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:961633/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:94531/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:906058/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:8727/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:869163/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:825048/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:799640/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:798039/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:750443/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:740291/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:593148/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:73528/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:712211/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:695592/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:69428/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:689066/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:674294/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:668618/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:654781/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:644490/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:640339/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1169264/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:187121/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1256708/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1250105/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1249606/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1233920/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1230418/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1217098/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1211537/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1192468/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1192183/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1172788/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1280613/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1132164/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1127448/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1114860/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1112304/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1107184/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1105712/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1084148/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:106417/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1049076/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1024238/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1432591/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:171153/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:166001/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:100177/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:157858/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1546317/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:153889/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1517670/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:148592/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1472461/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1465699/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:129589/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1402226/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:135773/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:134049/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1333219/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1325185/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:132287/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1306448/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1303272/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:129763/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGAGATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1210728/71‑1 (MQ=255)                                                 cGGCAGCGAATGGAATAGCGCTTTTCCGGCCTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAc  <  1:1523701/71‑1 (MQ=255)                                                 |                                                                       CAGCACCGATTCCCAGTGCGCGGTCATGTCCGGTCGCGTCGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAAC  >  minE/1172918‑1173036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑