Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 195,616 C→T L71L (CTG→CTA)  yaeF ← predicted lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE195,6160CT100.0% 520.6 / NA 142L71L (CTG→CTA) yaeFpredicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/142);  total (0/142)

Only 100 of 142 total aligned reads displayed.

GAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCA  >  minE/195563‑195640
                                                     |                        
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:219342/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:721913/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:726249/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:285010/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:278213/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:269283/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1001153/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:257533/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:239282/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:708113/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:198962/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:195966/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:176248/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:164484/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1548351/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:798012/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1504405/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1484378/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:444772/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:603356/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:566548/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:562133/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:645560/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:541774/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:667824/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:676782/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:453239/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:817710/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:684052/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:39354/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:357711/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:34229/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:694475/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:695598/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:322332/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1107841/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1224601/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1475054/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1182393/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1179285/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1176496/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1170485/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1164907/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1138021/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:898022/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1094866/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:899437/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1065684/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1058028/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1050765/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:974090/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1014766/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:993774/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1250802/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1447621/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1443092/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:879656/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:893600/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1411246/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1360985/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1342426/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1330325/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1323508/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:129068/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1264938/71‑1 (MQ=255)
gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1242885/71‑1 (MQ=255)
 aaGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:692560/70‑1 (MQ=255)
 aaGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1310558/70‑1 (MQ=255)
  aGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:1088266/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:969473/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:655487/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:801086/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:80151/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:259537/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:829681/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1531793/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1001733/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1045050/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1087296/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1205157/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1434487/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1437167/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1468321/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1485750/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1516623/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:546267/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1541340/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:299499/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:315165/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:320925/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:324582/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:336956/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:400504/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:427888/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:51226/69‑1 (MQ=255)
         aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:516804/69‑1 (MQ=255)
         aaGACGCGGATTCAAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:1073197/69‑1 (MQ=255)
                   ttCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:523824/59‑1 (MQ=255)
                                    ccAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt         <  1:971716/35‑1 (MQ=255)
                                     cAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCa  <  1:849793/41‑1 (MQ=255)
                                                     |                        
GAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCA  >  minE/195563‑195640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑