Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,509,579 G→A A42T (GCA→ACA)  ackA → acetate kinase A and propionate kinase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,509,5790GA100.0% 250.2 / NA 70A42T (GCA→ACA) ackAacetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (70/0);  total (70/0)

TTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGG  >  minE/1509564‑1509634
               |                                                       
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAAt                            >  1:1181076/1‑45 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGGGGCttaa          >  1:192227/1‑61 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:445926/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:565817/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:516669/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:496265/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:495460/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:482059/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:466033/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:46024/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:586419/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:445379/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:439748/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:423270/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:412403/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:380139/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:372090/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:348379/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:72720/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:916999/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:912724/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:878164/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:866220/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:778117/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:774848/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:767893/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:75007/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:568978/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:695287/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:691862/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:648465/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:638274/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:633580/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:626963/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:341815/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:143520/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1064951/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:106819/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1082994/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1191047/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1200210/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1216992/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1225922/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1255898/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:126849/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1315460/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1321800/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1385100/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1400419/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1000121/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1488767/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:229436/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:295631/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:289497/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:262571/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:251325/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:23208/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1530136/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:211438/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:163858/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1561289/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1551335/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1538842/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg  >  1:1538012/1‑71 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAg   >  1:674938/1‑70 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAg   >  1:1361865/1‑70 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAg   >  1:287125/1‑70 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAg   >  1:1080666/1‑70 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAg   >  1:897988/1‑70 (MQ=255)
ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGAGCAgg  >  1:265444/1‑71 (MQ=255)
               |                                                       
TTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGG  >  minE/1509564‑1509634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑