Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,763,736	C→T	noncoding (108/1542 nt)	rrsG ←	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,763,736	0	C	T	100.0%	16.2 / NA	39	noncoding (108/1542 nt)	rrsG	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/39);  total (0/39)

CATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTG  >  minE/1763722‑1763786               |                                                   taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:738556/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:350950/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:413511/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:422890/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:483907/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:503469/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:613234/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:613558/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:663113/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:694970/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:342943/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:743007/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:774262/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:800131/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:812711/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:816414/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:879245/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:884642/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:994722/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:9997/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1415950/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1047380/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1091134/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1198768/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1208455/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1218024/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1281600/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1283422/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1361268/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1035923/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1445006/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1492900/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:177187/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:190928/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:230091/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:307597/58‑1 (MQ=2) taTTACTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:318786/58‑1 (MQ=2)      cTCACCCGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1498990/54‑1 (MQ=2)          cccGTTCGCCACTCGTCAGCG‑‑G‑AGCAAGCT‑C‑‑CCTGTTACCGTTCGACTTg  <  1:1082571/50‑1 (MQ=2)               |                                                   CATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTG  >  minE/1763722‑1763786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑