Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,784,184	T→C	I223T (ATA→ACA)	yfjB →	NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,784,184	0	T	C	100.0%	71.3 / NA	21	I223T (ATA→ACA)	yfjB	NAD kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/0);  total (21/0)

TACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAA  >  minE/1784158‑1784229                           |                                              tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg     >  1:477939/1‑69 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:156554/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:973064/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:828302/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:737217/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:672370/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:281871/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:249763/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:235635/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:1034877/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:1507950/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:1494366/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:1384031/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:1337579/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:121251/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:1157489/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:1106325/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:1082316/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt    >  1:1047505/1‑70 (MQ=255) tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCAGTCTGCGTTTTTCGCATCGCCGt    >  1:330171/1‑70 (MQ=255) tACGTTGTCAGCACGACCACT‑GTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa  >  1:591997/1‑71 (MQ=255)                           |                                              TACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAA  >  minE/1784158‑1784229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑