Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	197,154	G→C	V149M (GTC→CTG)	proS ←	prolyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	197,154	0	G	C	100.0%	26.5 / NA	9	V149M (GTC→CTG)	proS	prolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (9/0);  total (9/0)

TCATCAGGAATTCGCGGGAACGCATGACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTG  >  minE/197129‑197195                          |                                          tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCt   >  1:674107/1‑66 (MQ=255) tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg  >  1:1095652/1‑67 (MQ=255) tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg  >  1:1124433/1‑67 (MQ=255) tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg  >  1:1158123/1‑67 (MQ=255) tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg  >  1:1478825/1‑67 (MQ=255) tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg  >  1:537409/1‑67 (MQ=255) tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg  >  1:542404/1‑67 (MQ=255) tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg  >  1:588324/1‑67 (MQ=255) tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg  >  1:682912/1‑67 (MQ=255)                          |                                          TCATCAGGAATTCGCGGGAACGCATGACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTG  >  minE/197129‑197195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑