Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,253,576	T→C	A1154S (GCA→TCG)	rpoC ←	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,253,576	0	T	C	100.0%	217.9 / NA	61	A1154S (GCA→TCG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (61/0);  total (61/0)

TACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTC  >  minE/2253539‑2253602                                      |                           tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:618349/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:315837/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:384778/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:392956/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:396522/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:396910/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:428933/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:431962/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:451844/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:467668/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:472472/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:55589/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:558322/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:559354/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:593106/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:605391/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:290362/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:640523/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:653288/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:67096/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:680994/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:731581/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:743374/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:819831/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:873065/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:878326/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:892227/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:91131/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:912268/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:949058/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:954339/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1231019/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1017900/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1022366/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1041474/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1078902/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:10838/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1085533/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1088186/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1094653/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:111140/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1156343/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1182564/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1183463/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1204153/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1010463/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1241988/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1331018/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1383415/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1412981/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1425247/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:146027/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1476340/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1548965/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:1556410/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:178938/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:191380/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:19999/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:202026/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGCTGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:282096/3‑64 (MQ=255) tgccGAAGGAGATGGTGCCGATGATTTCCGCCAGGATCGAAGGCTCTTTCGGACGACGAGCCTc  >  1:116/3‑64 (MQ=255)                                      |                           TACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTC  >  minE/2253539‑2253602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑