Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,450,298	A→G	W78R (TGG→CGG)	yifE ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,450,298	0	A	G	100.0%	40.3 / NA	13	W78R (TGG→CGG)	yifE	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/10);  total (3/10)

GAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTT  >  minE/2450246‑2450330                                                     |                                 gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:1081565/60‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:1180549/60‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:1279476/60‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:206902/60‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:273566/60‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:337862/60‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:377394/60‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:62152/60‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:71345/60‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt                           <  1:735603/60‑1 (MQ=255)               aCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGtt  >  1:594900/1‑71 (MQ=255)               aCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGtt  >  1:833117/1‑71 (MQ=255)               aCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGtt  >  1:916407/1‑71 (MQ=255)                                                     |                                 GAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTT  >  minE/2450246‑2450330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑