Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,516,656	C→T	A102T (GCT→ACT)	trmH ←	tRNA (Guanosine‑2'‑O‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,516,656	0	C	T	100.0%	134.7 / NA	38	A102T (GCT→ACT)	trmH	tRNA (Guanosine‑2'‑O‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/38);  total (0/38)

ATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGA  >  minE/2516633‑2516703                        |                                                aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:637961/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:238531/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:300252/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:338796/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:382902/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:414072/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:514503/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:557369/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:577296/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:57968/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:223445/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:680363/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:718119/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:719990/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:902652/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:909213/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:910094/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:949288/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:956539/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:218281/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1117833/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1241705/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1269968/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:134161/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1348884/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1355926/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1379377/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:142655/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1432379/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1441623/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1454037/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1500743/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1511046/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1517009/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1545468/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:176965/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1028699/71‑1 (MQ=255)  tCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga  <  1:1087162/70‑1 (MQ=255)                        |                                                ATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGA  >  minE/2516633‑2516703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑