Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,662,057	T→C	T258A (ACG→GCA)	rtcR ←	sigma 54‑dependent transcriptional regulator of rtcBA expression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,662,057	0	T	C	100.0%	163.3 / NA	46	T258A (ACG→GCA)	rtcR	sigma 54‑dependent transcriptional regulator of rtcBA expression
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/0);  total (46/0)

AACGCGCCTTTTACATGACCAAACAGCGTCGACATGGCGGTATCGCCGCGCAGGGTGGCGCAGTTCACTTC  >  minE/2662029‑2662099                             |                                           aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:771791/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:339710/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:35175/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:404357/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:404535/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:453722/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:455975/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:555926/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:650201/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:694692/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:730647/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:757236/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:283715/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:809739/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:824738/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:863554/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:864989/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:888574/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:898025/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:913595/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:924173/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:974876/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:997048/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1431963/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1086725/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1110253/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1131188/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1285323/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1288228/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1324965/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1342221/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1342859/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1361041/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1366329/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1417682/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1006492/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1446799/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1487482/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1542430/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1547943/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:188011/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:195811/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:213257/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:227824/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:240710/4‑71 (MQ=255) aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:274650/4‑71 (MQ=255)                             |                                           AACGCGCCTTTTACATGACCAAACAGCGTCGACATGGCGGTATCGCCGCGCAGGGTGGCGCAGTTCACTTC  >  minE/2662029‑2662099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑