Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,726,174 G→A R303R (CGC→CGT)  yheS ← fused predicted transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,726,1740GA98.7% 268.3 / ‑5.7 75R303R (CGC→CGT) yheSfused predicted transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (74/0);  minor base T (1/0);  total (75/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGG  >  minE/2726108‑2726177
                                                                  |   
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGcgcacgc  >  1:182984/1‑69 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGcgcacg   >  1:100116/1‑69 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGcgcacg   >  1:1141456/1‑69 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCTCgg  >  1:338783/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:374750/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:605666/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:593985/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:557919/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:552835/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:484306/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:45998/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:428818/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:418512/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:415434/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:401737/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:632413/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:344914/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:341444/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:338972/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:331572/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:253025/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:842748/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:987731/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:968025/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:967038/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:962207/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:942612/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:908432/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:906426/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:898696/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:854967/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:625761/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:834207/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:799447/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:788077/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:780605/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:767215/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:756169/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:673833/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:654385/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:196028/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1166005/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1331107/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1294652/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1272860/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1250695/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1244683/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1215891/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1187934/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1167275/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1346330/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1149642/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1137243/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1073119/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1046070/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1034200/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1020698/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1018166/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1010140/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1513052/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:184790/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:180965/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:176990/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:170005/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:16728/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:162235/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1537807/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1517088/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:215294/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1464734/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1459723/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1458147/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1451407/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1450015/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1444524/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1428547/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg  >  1:1395258/1‑70 (MQ=255)
gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCGTTCCGGCGCACgg  >  1:1142935/1‑70 (MQ=255)
                                                                  |   
GCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGG  >  minE/2726108‑2726177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑